Epigallocatechin Gallate-Mediated Alteration of the MicroRNA Expression Profile in 5α-Dihydrotestosterone-Treated Human Dermal Papilla Cells.
- Author:
Shanghun SHIN
1
;
Karam KIM
;
Myung Joo LEE
;
Jeongju LEE
;
Sungjin CHOI
;
Kyung Suk KIM
;
Jung Min KO
;
Hyunjoo HAN
;
Su Young KIM
;
Hae Jeong YOUN
;
Kyu Joong AHN
;
In Sook AN
;
Sungkwan AN
;
Hwa Jun CHA
Author Information
- Publication Type:Original Article
- Keywords: Epigallocatechin gallate; Human dermal papilla cells; MicroRNAs
- MeSH: Aging; Alopecia; Cell Cycle Checkpoints; Cell Death; Cell Survival; Computational Biology; Dihydrotestosterone; Genome; Hair; Hair Follicle; Humans*; MicroRNAs*; Reactive Oxygen Species; Tea
- From:Annals of Dermatology 2016;28(3):327-334
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Dihydrotestosterone (DHT) induces androgenic alopecia by shortening the hair follicle growth phase, resulting in hair loss. We previously demonstrated how changes in the microRNA (miRNA) expression profile influenced DHT-mediated cell death, cell cycle arrest, cell viability, the generation of reactive oxygen species (ROS), and senescence. Protective effects against DHT have not, however, been elucidated at the genome level. OBJECTIVE: We showed that epigallocatechin gallate (EGCG), a major component of green tea, protects DHT-induced cell death by regulating the cellular miRNA expression profile. METHODS: We used a miRNA microarray to identify miRNA expression levels in human dermal papilla cells (DPCs). We investigated whether the miRNA expression influenced the protective effects of EGCG against DHT-induced cell death, growth arrest, intracellular ROS levels, and senescence. RESULTS: EGCG protected against the effects of DHT by altering the miRNA expression profile in human DPCs. In addition, EGCG attenuated DHT-mediated cell death and growth arrest and decreased intracellular ROS levels and senescence. A bioinformatics analysis elucidated the relationship between the altered miRNA expression and EGCG-mediated protective effects against DHT. CONCLUSION: Overall, our results suggest that EGCG ameliorates the negative effects of DHT by altering the miRNA expression profile in human DPCs.
