Ampelopsis japonica Makino Extract Inhibits the Inflammatory Reaction Induced by Pathogen-Associated Molecular Patterns in Epidermal Keratinocytes.
- Author:
Mi Ra CHOI
1
;
Dae Kyoung CHOI
;
Ki Duck KIM
;
Sue Jeong KIM
;
Dong Il KIM
;
Myung IM
;
Young LEE
;
Young Joon SEO
;
Chang Deok KIM
;
Jeung Hoon LEE
Author Information
- Publication Type:Original Article
- Keywords: Ampelopsis; Inflammation; Keratinocytes; Pathogen-associated molecular pattern molecules
- MeSH: Ampelopsis*; Cytokines; Dermatitis; Epidermis; Hyperplasia; Immunity, Innate; Inflammation; Interleukin-6; Interleukin-8; Interleukins; Keratinocytes*; Necrosis; Nucleotides; Pathogen-Associated Molecular Pattern Molecules*; RNA; Skin
- From:Annals of Dermatology 2016;28(3):352-359
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Keratinocytes are the major cells in epidermis, providing barrier components such as cornified cells through the sophisticated differentiation process. In addition, keratinocytes exerts their role as the defense cells via activation of innate immunity. It has been known that pathogen-associated molecular patterns (PAMPs) including double-strand RNA and nucleotides can provoke inflammatory reaction in keratinocytes. OBJECTIVE: The aim of this study is to evaluate the effect of Ampelopsis japonica Makino extract (AE) on PAMPs-induced inflammatory reaction of keratinocytes. METHODS: The effects of AE were determined using poly (I:C)-induced inflammation and imiquimod-induced psoriasiform dermatitis models. RESULTS: In cultured keratinocytes, AE significantly inhibited poly(I:C)-induced expression of inflammatory cytokines, such as interleukin (IL)-1β, IL-6, IL-8 and tumor necrosis factor-α. AE significantly inhibited poly(I:C)-induced release of caspase-1 active form (p20), and down-regulated nuclear factor-κB signaling pathway. In imiquimod-induced psoriasiform dermatitis model, topical application of AE resulted in significant reduction of epidermal hyperplasia. CONCLUSION: These results suggest that AE may be a potential candidate for the treatment of skin inflammation.
