Preparation and application of novel photosensitive nanoparticles in tumor photodynamic therapy
10.12025/j.issn.1008-6358.2024.20240435
- VernacularTitle:新型光敏纳米粒子的制备及其在肿瘤光动力治疗中的应用
- Author:
Mengzhou GUO
1
;
Huan LIU
2
;
Tong LI
3
;
Yiyi YU
1
Author Information
1. Department of Medical Oncology, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
2. Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.
3. Department of Hepatic Oncology, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
- Publication Type:Originalarticle
- Keywords:
photodynamic therapy;
photosensitizer;
nanoparticles;
reactive oxygen species;
tumor
- From:
Chinese Journal of Clinical Medicine
2024;31(4):577-585
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare a novel photosensitive nanoparticle and to evaluate its physicochemical properties, and effect on the efficacy of photodynamic therapy. Methods 5,15-dibromo-10,20-diphenylporphine (DBN), tetrafluoroterephthalonitrile (TFN), and the amphiphilic polymer methoxy-polyethylene glycol-distearoylphosphatidylethanolamine (DSPE-MPEG2000, PEG) were dissolved in tetrahydrofuran (THF) by co-precipitation method to prepare novel photosensitive nanoparticles, named DBN/TFN@PEG. The physicochemical properties of DBN/TFN@PEG were characterized. Both novel and conventional nanoparticles were continuously irradiated with a 660 nm laser, and the fluorescence intensity of nanoparticles, representing reactive oxygen species (ROS) production levels, was measured using a fluorescence spectrophotometer at different irradiation times. Tumor cells were co-incubated with the nanoparticles and irradiated with a 660 nm laser. ROS levels within the tumor cells were detected using immunofluorescence, and the ratio of dead to live tumor cells was determined using PI/Calcein-AM staining. Results Prepared DBN/TFN@PEG nanoparticles with hydrated particle size of approximately 107.8 nm were uniformly distributed in the solution. Compared to conventional nanoparticles, the ROS production capacity of DBN/TFN@PEG was significantly higher (P＜0.01). Immunofluorescence results showed that the generation of ROS levels in the tumor cells of DBN/TFN@PEG group were significantly higher than in the conventional nanoparticles group under laser irradiation (P＜0.01). PI/Calcein-AM staining results indicated a significantly higher ratio of dead tumor cells in the DBN/TFN@PEG group compared to the conventional nanoparticle group (P＜0.01). Conclusions DBN/TFN@PEG has stable physicochemical properties and uniform distribution in the solution. As effective photosensitizers, DBN/TFN@PEG can exhibit stronger ability to induce ROS generation in tumor cells, and may enhance the efficacy of photodynamic therapy in cancer.
