Th17/Treg related cytokine imbalance participates in the pathogenesis of preeclampsia
10.19405/j.cnki.issn1000-1492.2023.07.022
- Author:
Siyu Xie
1
;
Ying Zhang
1
;
Fan Chen
1
Author Information
1. Dept of Obstetrics and Gynecology , The First Afiliated Hospital of Anhui Medical University , Hefei 230022
- Publication Type:Journal Article
- Keywords:
preeclampsia;
Th17/Treg;
IL⁃10;
IL⁃23;
IL⁃2R
- From:
Acta Universitatis Medicinalis Anhui
2023;58(7):1194-1199
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the role of T cell 17/regulatory T cell (Th17/Treg) related cytokine imbalance
in the pathogenesis of preeclampsia .
Methods :Serum samples from 50 preeclampsia pregnant women and 30 normal pregnant women were collected , and the concentration of interleukin⁃23(IL⁃23)and interleukin⁃2 recepter(IL⁃2R) in serum was tested using ELISA . Additionally , the concentration of interleukin⁃6( IL⁃6) and interleukin⁃10( IL⁃10)in serum was measured using cell microsphere array(CBA) technology. Then placental and decidual tissues of 10 pregnant women with preeclampsia and 6 normal pregnant women were selected for further verification of the expression of related cytokines by using QPCR and immunohistochemistry .
Results :The concentrations of IL⁃10 , IL⁃2R , IL⁃23 and IL⁃6 in the serum of preeclampsia pregnant women were higher than those of normal pregnant women (P < 0. 05) . The QPCR results showed that , compared to normal pregnant women , there was no statisticly significant difference in the expression of IL⁃23 mRNA in the placenta and decidua tissues of preeclampsia patients (P > 0. 05) , while the expression level of IL⁃10 mRNA in the placenta and decidua tissues of preeclampsiapatients was lower than that of normal pregnant women , with a statistically significant difference (P < 0. 05) . The expression level of IL⁃2R mRNA in the placenta and decidua tissues of preeclampsia patients was higher than that of normal pregnant women , with a statistically significant difference ( P < 0. 05 ) . The immunohistochemical results showed that the expression of IL⁃10 in the placenta and decidua of preeclampsia pregnant women was lower than that of normal pregnant women , with a statistically significant difference (P < 0. 05) . The expression of IL⁃2R and IL⁃23 in the placenta of preeclampsia was higher difference (P < 0. 05) . However , there was no statistically significant difference in the expression of IL⁃23 and IL⁃than that of normal pregnant women , with a statistically significant 2R in the decidua of the two groups (P > 0. 05) .
Conclusion :Compared with normal pregnant women , there are differences in the expression levels of IL⁃10 , IL⁃23 , and IL⁃2R in preeclampsia pregnant women . This difference may be related to Th17/Treg cellular immune imbalance , suggesting that Th17/Treg immune imbalance mediated
changes in related cytokines may be involved in the pathogenesis of preeclampsia .
- Full text:202410151044081617Th17_Treg相关细胞...失衡参与子痫前期的发病机制_谢丝雨.pdf
