The association between RABL6 gene methylation in peripheral blood and early lung cancer in Chinese population
- Author:
Haixia Huang
1
;
Rong Qiao
2
;
Mengxia Li
1
;
Wanjian Gu
3
;
Baohui Han
2
;
Rongxi Yang
1
Author Information
- Publication Type:Journal Article
- Keywords: lung cancer; DNA methylation; RABL6; peripheral blood; biomarker
- From: Acta Universitatis Medicinalis Anhui 2023;58(6):1030-1036
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To explore the relationship between the methylation level of RABL6 in peripheral blood and early lung cancer (LC) with a case-control study in the Chinese population．
Methods :The methylation levels of 7 CpG sites in RABL6 gene in peripheral blood of samples from 275 LC patients (81．5% at stage I) ，and age- and gender-matched 185 benign lung nodule cases and 267 matched healthy controls were measured by matrix-assisted laser desorption ionization time-of-flight mass spectrometry．Multinomial Logistic regression adjusted for covariates was used to analyze the association between the RABL6 methylation and LC．Mann-Whitney U test was applied for the comparisons of RABL6 methylation levels between clinical characteristics subgroups of LC.
Results :Compared to the healthy controls，the methylation of RABL6 _CpG_ 17 was inversely associated with LC in females ( per - 10% methylation : OR = 2. 47，95% CI = 1. 19－5. 13，P = 0. 016) ，but positively associated with LC in males (per - 10% methylation : OR = 0. 52，95% CI = 0. 29－0. 94，P = 0. 030) ．In addition，hypermethylation of RABL6_CpG _2 and RABL6_CpG_5 was significantly associated with LC in the subjects older than 55 years (for RABL6_CpG_ 2 : per －10% methylation : OR = 0. 77，95% CI = 0. 60－0. 99，P = 0. 038 ; for RABL6_CpG_5 : OR = 0. 58，95% CI = 0. 34－0. 97，P = 0. 038) ．
Conclusion :The study reveals an association between peripheral blood-based RABL6 methylation levels and early LC，providing a new clue for developing peripheral blood-based DNA methylation as a potential marker for the evaluation of LC risk. - Full text:2024101510060073729中国人群外周血RABL6基...基化与早期肺癌的关联性研究_黄海霞.pdf
