Preliminary study on the expression and function of bone morphogenetics protein 7 in ESCC

Mengfei Sun; Hongfang Huang; Yuhang Dong; Huakun Zhang; Ziru Zhou; Qi Sun; Wenyan Guan; Linyue Zhao; Xiaobin Cui; Yunzhao Chen; Feng Li

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Preliminary study on the expression and function of bone morphogenetics protein 7 in ESCC

Author: Mengfei Sun ¹ ; Hongfang Huang ¹ ; Yuhang Dong ¹ ; Huakun Zhang ¹ ; Ziru Zhou ¹ ; Qi Sun ² ; Wenyan Guan ² ; Linyue Zhao ² ; Xiaobin Cui ² ; Yunzhao Chen ^{3
,

4} ; Feng Li ¹
Author Information

1. Dept of Pathology，School of Medicine，Shihezi University，Shihezi 832002
2. Dept of Pathology，Nanjing Drum Tower Hospital，The Affiliated Hospital of Nanjing University Medical School，Nanjing 210008
3. Dept of Pathology，The People '
4. s Hospital of SND，Suzhou 215129
Publication Type:Journal Article
Keywords: esophageal squamous cell carcinoma; BMP7; immunity; prognosis
From: Acta Universitatis Medicinalis Anhui 2023;58(6):995-1000,1010
CountryChina
Language:Chinese
Abstract: Objective : To analyze the expression and immune infiltration levels of the BMP7 gene ( BMP7) in e- sophageal squamous cell carcinoma(ESCC) ．
Methods :Initially，in 274 cases of ESCC and 242 cases of normal tissues，the level of BMP7 was verified by immunohistochemistry ，and the relationship between the expression difference and the survival cycle and clinical pathological characteristics of patients with ESCC was explored，and BMP7 overexpression plasmid transfection of ESCC cells was established，and the effect of BMP7 on the biological behavior of ESCC cells was examined by CCK-8，Clone，and Transwell.
Results : BMP7 expression in normal e- sophageal tissues was higher than that of ESCC(P＜0. 001) ，the expression level of BMP7 was correlated with the degree of differentiation of patients(P = 0. 006) and TNM staging(P ＜0. 001) ，and the survival of patients with high expression of BMP7 exceeded that of patients with low BMP7 (P = 0. 041) ，and the experiments of CCK-8 and Clone showed that the proliferation effect of cells in the overexpressed BMP7 group was lower than that of the control group．Transwell experiments confirmed that the cell invasion migration capacity of the overexpressed BMP7 group was less than that of the control one．The immune infiltration results showed that BMP7 was positively correlated with macrophages(P = 0. 008) and negatively correlated with γ-δT cells(P = 0. 028) ．
Conclusion : BMP7 is low in ESCC and associated with poor prognosis and immune infiltration levels in patients.
Full text:2024101509130260965骨形成蛋白7在ESCC中的表达及功能初步探究_孙梦菲 (1).pdf