Advances in the differentiation of human induced pluripotent stem cells into osteoblasts
10.12016/j.issn.2096-1456.202440158
- Author:
LIAO Lingzi
1
;
SONG Yameng
1
;
LIU Meixuan
1
;
LI Siyi
1
;
ZHOU Ping
2
Author Information
1. School of Stomatology, Lanzhou University
2. 1.School of Stomatology, Lanzhou University 2.Gansu Provincial Key Laboratory of Dental and Maxillofacial Reconstruction and Biological Intelligent Manufacturing
- Publication Type:Review
- Keywords:
bone regeneration / human induced pluripotent stem cells / osteoblast / induced differentiation / embryoid bodies method / monolayer culture method / defined stepwise differentiation method / three-dimensional orientation / induction system
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2024;32(10):805-813
- CountryChina
- Language:Chinese
-
Abstract:
Bone diseases, such as osteoporosis and osteoarthritis, have emerged as pressing public health concerns requiring immediate attention and resolution. Cellular therapy and tissue engineering techniques are among the most promising therapeutic approaches for such conditions. Human induced pluripotent stem cells (hiPSCs) possess remarkable capacity for indefinite self-renewal in vitro and the ability to differentiate into all somatic cell types originating from the three germ layers, thereby making them a promising source of osteoblasts. Consequently, it is crucial to establish a well-delineated system for osteogenic differentiation of hiPSCs in vitro, with the aim to generate osteoblast-like cells that conform to clinical application standards. Numerous research teams have achieved substantial advancements in both the direct osteogenic differentiation of hiPSCs and the indirect pathway via mesenchymal stem cells. In this article, we provide a comprehensive review of these two osteogenic differentiation pathways and their current applications, with the aim of serving as a valuable reference for bone regeneration technologies. Current research efforts have relied on embryoid body formation and monolayer induction methods utilizing biomaterials to develop a system that facilitates in vitro culture and osteogenic differentiation of hiPSCs. However, the existing research is primarily constrained by unclear system components and low efficiency. Therefore, the development of a stepwise and three-dimensional induction system based on stringent regulation by specific compounds is a primary research direction for the future.
- Full text:2024101416444733358人诱导多能干细胞成骨分化的研究进展.pdf
