Content determination and chemometric analysis of 8 components in Smilax glabra
- VernacularTitle:土茯苓中8个成分的含量测定及化学计量学分析
- Author:
Huiliang ZOU
1
;
Xincai WANG
1
;
Ke CHEN
1
;
Hong CUI
1
;
Lingjie MENG
2
;
Bailian LIU
3
;
Min CHEN
1
Author Information
1. Huzhou Institute for Food and Drug Control，Zhejiang Huzhou 313000，China
2. Institute of Life Sciences，Zunyi Medical University，Guizhou Zunyi 563000，China
3. Institute of Traditional Chinese Medicine and Natural Products，College of Pharmacy，Jinan University，Guangzhou 510632，China
- Publication Type:Journal Article
- Keywords:
Smilax glabra;
chemometrics;
content determination;
UPLC;
5-O-caffeoylshikimic acid;
astilbin;
isoastilbin
- From:
China Pharmacy
2024;35(19):2378-2382
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To provide reference for Smilax glabra quality control. METHODS UPLC method was established to simultaneously determine the contents of 5-O-caffeoylshikimic acid， neoastilbin， astilbin， neoisoastilbin， isoastilbin， engelitin， resveratrol and isoengelitin in 20 batches of S. glabra from different areas （No. S1-S20）. The quality evaluation of 20 batches of samples was performed by chemometrics； the differential biomarkers that affected the quality of S. glabra were screened. RESULTS The measured 8 components had good linear relationship within the range of measured concentration （r≥0.999 6）. RSDs of precision， repeatability and stability tests （24 h） were all lower than 2.00% （n＝6）. The average recoveries varied between 97.60% and 106.40% （RSDs were all lower than 2.00%， n＝6）. Cluster analysis showed that the samples produced in Zhejiang （S1-S5） and Jiangxi （S6-S10） were clustered into one category； the samples produced in Hunan （S11-S15） were clustered into one category； the samples produced in Yunnan （S16-S20） were clustered into one category. Principal component analysis showed that the first two principal components could represent 85.60% information of 8 components in S. glabra. Partial least squares-discriminant analysis showed that variable importance projection values of 5-O-caffeoylshikimic acid， astilbin， isoastilbin and neoastilbin were all greater than 1. CONCLUSIONS There are differences in the contents of the above 8 components in S. glabra from different origins； 5-O-caffeoylshikimic acid， astilbin， isoastilbin and neoastilbin may be differential markers affecting the quality of S. glabra.
