Study on pharmacodynamic substances of anti-inflammatory effect of Zhuang medicine Stahlianthus involucratus based on metabolism in rats
- VernacularTitle:基于大鼠体内代谢的壮药三七姜抗炎药效物质基础研究
- Author:
Xingchen LIU
1
,
2
,
3
;
Jie LIANG
1
,
2
,
3
;
Chunyan HUANG
4
;
Jiayi CHEN
1
,
2
;
Jiawen PENG
1
,
2
;
Jingjing XIE
1
,
2
;
Yuming MA
1
,
2
;
Sisi CHEN
1
,
2
;
Jiali WEI
1
Author Information
1. College of Pharmacy，Guangxi University of Chinese Medicine/ Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine，Nanning 530200，China
2. Collaborative Innovation Center of Zhuang and Yao Ethnic Medicine，Nanning 530200，China
3. Guangxi Zhuang Autonomous Region Ethnic Medicine Resources and Application Engineering Research Center，Nanning 530200，China
4. Qinzhou Inspection and Testing Center，Guangxi Qinzhou 535000，China
- Publication Type:Journal Article
- Keywords:
Stahlianthus involucratus;
chemical constituents;
pharmacodynamic substances;
internal metabolism;
inflammation
- From:
China Pharmacy
2024;35(19):2358-2364
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To provide reference for basic analysis of the pharmacodynamic substance in Stahlianthus involucratus. METHODS Overall 24 SD male rats were randomly divided into blank group （purified water）， and administration group （ethanol extract of S. involucratus， 15.75 g/kg， calculated by crude drug）， with 12 rats in each group. They were given drug liquid/purified water intragastrically， twice a day， every 6-8 h， for consecutive 3 days. After medication， the blood， urine and fecal samples were collected from two groups of rats. UPLC-Q-Exactive-MS technology was used to identify the chemical constituents in the ethanol extract of S. involucratus， and metabolites in the blood， urine and fecal of rats after intragastrical administration of the ethanol extract of S. involucratus. Multivariate statistical analysis was employed to screen various serum metabolites. Metabolic pathways were analyzed by MetaboAnalyst 5.0 platform. RESULTS A total of 38 chemical constituents were identified from the ethanol extract of S. involucratus， including fourteen prototype components and three metabolites identified from 5 urine samples， nine prototype components identified from fecal samples， and ten prototype components and one metabolite identified from serum samples. A total of 71 differential metabolites were screened from two groups of rat serum samples， of which 44 differential metabolites， such as ferulic acid， glycyrrhizin， were up-regulated and 27 differential metabolites， such as arachidonic acid， phenylacetylglutamine， were down-regulated. The 71 differential metabolites were mainly enriched in 11 metabolic pathways， including phenylalanine metabolism， linoleic acid metabolism， arachidonic acid metabolism， and tryptophan metabolism. CONCLUSIONS Ferulic acid， liquiritigenin， isofraxidin and formononetin may be the material basis that directly exert pharmacological effects of S. involucratus. S. involucratus may exert anti-inflammatory effects by affecting metabolic pathways， including arachidonic acid metabolism and tryptophan metabolism.
