Expression and clinical significance of vascular and vasculogenic mimicry generation factors VEGFA and MMP⁃14 in lung adenocarcinoma
10.19405/j.cnki.issn1000-1492.2023.07.018
- Author:
Hui Li
1
,
2
;
Junfeng Huang
3
;
Jing Zhou
3
;
Tingdong Zhou
3
;
Liwen Chen
3
Author Information
1. Dept of Clinical Laboratory , The Second Afiliated Hospital of Anhui Medical University , Hefei 230601
2. Dept of Clinical Laboratory , Fuyang Hospital of Anhui Medical University , Fuyang 236112
3. Dept of Clinical Laboratory , The Second Afiliated Hospital of Anhui Medical University , Hefei 230601
- Publication Type:Journal Article
- Keywords:
LUAD;
vascular endothelial growth factor A;
matrix metalloproteinase 14;
diagnostic efficacy;
bioin⁃ formatic analysis;
survival prognosis
- From:
Acta Universitatis Medicinalis Anhui
2023;58(7):1171-1177
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To investigate the expression and clinical significance of classic vascular endothelial growth factor A (VEGFA) and vasotropic mimicry factor matrix metalloproteinase 14 (MMP⁃14) in lung adenocarcinoma(LUAD) .
Methods : The expression levels of MMP⁃14 and VEGFA genes and proteins in LUAD and their correlation with survival and prognosis were analyzed using TCGA and UALCAN databases . Serum of 69 patients with LUAD and 20 healthy subjects (control group) was collected , and the contents of MMP⁃14 and VEGFA were detected by ELISA and chemiluminescence , respectively , to analyze the correlation between the two and the clinicopathological features of tumors and their value in prediction and diagnosis of LUAD .
Results: The levels of MMP⁃14 and VEGFA in LUAD tissues and serum were higher than those in control group . There were significant differences in serum VEGFA and MMP⁃14 expression levels among early stage group , advanced stage group and control group (P< 0. 001) . MMP⁃14 was higher in T3 /T4 stage than that in T1 /T2 stage (P = 0. 045) , higher in N2 /N3 stage than that in N0 /N1 stage (P = 0. 035) , and higher in the pleural metastasis group than that in the non⁃pleural metastasis group (P = 0. 034) . VEGFA level was higher in M 1 than M0 (P = 0. 025) . Elevated VEGFA level was a risk factor for LUAD (P = 0. 002) . The area under the curve (AUC) of MMP⁃14 , VEGFA and CEA alone was 0. 793 , 0. 849 and 0. 851 , respectively , and the AUC of the combined test was 0. 952 . The overall survival ( OS) and disease specific survival (DSS) of MMP⁃14 and VEGFA low expression group were longer than those of MMP⁃14 and VEGFA high expression group (P < 0. 05) .
Conclusion : High expression of MMP⁃14 and VEGFA in LUAD is associated with the growth , invasion and metastasis of LUAD , respectively , and has implications for survival prognosis determination .
- Full text:2024101016473210060促血管和血管拟态生成因子V...在肺腺癌中的表达及临床意义_李辉.pdf
