Mast cells regulate inflammatory bowel disease by mediating macrophage polarization
10.19405/j.cnki.issn1000-1492.2023.07.015
- Author:
Tianyu Yu
1
,
2
,
3
,
4
,
5
;
Daoli Liu
1
,
6
;
Zhaohui Wang
1
,
6
;
Shuangjiu Hu
1
,
6
;
Bin Liu
1
,
6
Author Information
1. Dept of Gastrointestinal Surgery,Anqing First People &prime
2. s Hospital of Anhui Medical University , Anqing 246000
3. Clinical Medical College of Anhui Medical University , Hefei 230000
4. Dept of General Surgery, Shanghai Tenth People &prime
5. s Hospital of Tongji University, Shanghai 200000
6. s Hospital of Anhui Medical University , Anqing 246000
- Publication Type:Journal Article
- Keywords:
mast cell;
inflammatory bowel disease;
macrophages;
inflammatory factors;
anti⁃inflammatory fac⁃ tors;
polarization
- From:
Acta Universitatis Medicinalis Anhui
2023;58(7):1152-1158
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the role of mast cell (MC) in the progression of inflammatory bowel disease
(IBD) and the possible regulatory mechanisms .
Methods :Wild type mice , MC⁃deficient mice and MC reconstituted mice were used to construct dextran sodium sulfate ( DSS) Ⅳinduced colitis models . Assays such as toluidine blue staining , flow cytometry and Real⁃time quantitative PCR were used to analyze results .
Results :MCs migrated and infiltrated to the intestine under the induction of stem cell factor (Scf) and matrix metalloprotein 9 (Mmp⁃9)factors . In the mouse IBD models , the integrity of intestinal mucosal villi in MC⁃deficient mice was worse than that in WT mice . In MC⁃deficient mice , the intestinal inflammation was more severe and the ability of mucosal repair was worse . After MC reconstruction , intestinal inflammation was reduced and mucosal repair ability was restored .
The ratio of macrophage M1 /M2 in WT mouse was lower than that in MC⁃deficient mouse . The genes interleukin⁃4(Il4) and interleukin⁃10 (Il10) associated with M2 macrophages were higher in the WT mouse than in the MC⁃deficient mouse , and other anti⁃inflammatory factor genes interleukin⁃13 ( Il13 ) and GATA binding protein 3 (Gata3) were also higher than those in the WT group . The pro⁃inflammatory factor gene inducible nitric oxide synthase(Inos) was opposite .
Conclusion :MC polarizes macrophages to exert anti⁃inflammatory and promote mucosal repair in the process of IBD .
- Full text:2024101016322202051肥大细胞调节巨噬细胞极化在炎症性肠病中的作用_余畋余.pdf
