Expression of programmed death 1 on CXCR5 - CD4 + T cell from the patients with systemic lupus erythematosus and clinical significance
10.19405/j.cnki.issn1000-1492.2023.07.008
- Author:
Qing Luo
1
;
Lu Zhang
1
;
Zikun Huang
1
;
Biqi Fu
2
;
Junming Li
1
Author Information
1. Dept of Clinical Laboratory, The First Afiliated Hospital ofNanchang University,Nanchang 330006
2. Dept ofRheumatology, The First Afiliated Hospital ofNanchang University,Nanchang 330006
- Publication Type:Journal Article
- Keywords:
systemic lupus erythematosus;
CXCR5 - CD4 + T cells;
PD1;
predictive model
- From:
Acta Universitatis Medicinalis Anhui
2023;58(7):1103-1110
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the expression of programmed death 1 (PD1) on CXCR5 - CD4 + T cells from the patients with systemic lupus erythematosus ( SLE) and to analyze the clinical relevance to disease severity.
Methods : The expression of PD1 on CXCR5 - CD4 + T cells was examined from 47 SLE patients and 35 healthy
controls (HC) by the technique of flow cytometry. The expression of PD1 including percentage of PD1 + CXCR5 -CD4 + T cells and mean fluorescence intensity (MFI) on CXCR5 - CD4 + T cells was compared between SLE patients and HC. And its correlation with clinical indicators , laboratory inspection and the percentage of plasmablasts was analyzed. Moreover, the predictive value of the expression of PD1 on CXCR5 - CD4 + T cell was explored.
Results:① The percentage of PD1 + CXCR5 - CD4 + T cells from SLE patients significantly elevated compared with HC (P= 0. 008 3 , U = 540. 5) , and the MFI of PD1 on CXCR5 - CD4 + T cells from SLE patients significantly elevated compared with HC (P < 0. 000 1 , U = 187. 0) . ② The percentage of PD1 + CXCR5 - CD4 + T cells was associated with C3 ( rs = - 0. 335 2 , P = 0. 022 8) , anti - SSA (P = 0. 016 6 , t = 2. 5) , anti - histone (P = 0. 030 3 , t =2. 3) , treatment (P = 0. 020 2 , t = 3. 4) , plasmablasts ( rs = 0. 387 1 , P = 0. 0072) in SLE patients. The MFI of PD1 on CXCR5 - CD4 + T cells was associated with SLEDAI ( rs = 0. 403 1 , P = 0. 005 0) , ESR ( rs = 0. 356 1 , P= 0. 017 7) , CRP ( rs = 0. 337 4 , P = 0. 028 9) , RBC ( rs = - 0. 297 0 , P = 0. 042 6) , HGB ( rs = - 0. 302 9 , P= 0. 038 5) , HCT ( rs = - 0. 381 6 , P = 0. 008 1) , L ( rs = - 0. 393 7 , P = 0. 006 2) , L% ( rs = - 0. 391 2 , P= 0. 006 5) , N% ( rs = 0. 315 0 , P = 0. 031 1) , NLR ( rs = 0. 373 0 , P = 0. 009 8) , LMR ( rs = - 0. 431 5 , P =0. 002 5) , dNLR ( rs = 0. 315 0 , P = 0. 031 1) , anti⁃Ro52 (P = 0. 029 5 , t = 63. 5) , treatment (P = 0. 035 5 , W= 21) , plasmablasts ( rs = 0. 315 8 , P = 0. 030 6) . ③ Logistic regression analysis showed that the MFI of PD1 on CXCR5 - CD4 + T cells was a risk factor for SLE. ④ ROC analysis showed the AUC of the MFI of PD1 on CXCR5 -CD4 + T cells was 0. 886. A further established model based on combination of the MFI of PD1 on CXCR5 - CD4 + T cells and HGB showed predictive value in distinguishing SLE from HC with AUC of 0. 979. And predictive value was positively associated with SLEDAI ( rs = 0. 313 6 , P = 0. 030 3) .
Conclusion :Increased percentage of PD1 + CXCR5 - CD4 + T cells and increased MFI of PD1 on CXCR5 - CD4 + T cells in SLE are associated with disease severity and activity , and a model based on combination of the MFI of PD1 on CXCR5 - CD4 + T cells and HGB shows prominent value for predicting SLE.
- Full text:2024100917065091826系统性红斑狼疮患者外周血C...+T细胞上PD1表达及意义_罗清.pdf
