Constructing transgenic mice to label hepatic stellate cells by Cre⁃Loxp technology
10.19405/j.cnki.issn1000-1492.2023.07.001
- Author:
Ziwei Huang
1
;
Dianyuan Zhao
2
,
3
;
Long Xu
4
;
Li Tang
3
,
4
,
5
Author Information
1. Dept of Immunology, School of Basic Medical Sciences , Anhui Medical University, Hefei 230032
2. Institute of Lifeomics ,Academy of Military Medical Sciences ,Academy ofMilitary Sciences ,Beijing 102206
3. State Key Laboratory of Proteomics ,Beijing Proteome Research Center, National Center for Protein Sciences ,Beijing 102206
4. Dept of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei 230032
5. Institute of Lifeomics ,Academy of Military Medical Sciences ,Academy ofMilitary Sciences ,Beijing 102206
- Publication Type:Journal Article
- Keywords:
hepatic stellate cells;
Kupffer cells;
hepatic sinusoidal endothelial cells;
reporter mouse;
Cre⁃LoxP
- From:
Acta Universitatis Medicinalis Anhui
2023;58(7):1065-1070
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To trace and investigate the distribution of hepatic stellate cells by Cre⁃Loxp technology of LratCre mice.
Methods :LratCre mice were mated with H11LoxP⁃ZsGreen⁃Stop⁃LoxP⁃tdTomato reporter mice and genotype of their progeny was identified by PCR. The liver sections were fixed , the antibody of liver non⁃parenchymal cells was labeled with immunofluorescence , and the expression and distribution of liver stellate cells and other liver non⁃parenchymal cells were analyzed by Imaris 3D reduction , as well as positively identify dual positioning of hepatic stellate cells.
Results : LratCre H11LoxP⁃ZsGreen⁃Stop⁃LoxP⁃tdTomato reporter mice specificiallyled to expression of red fluorescent protein tdTomato in hepatic stellate cells , providing a way to analyze the interactions between hepatic stellate cells , Kupffer cells and liver sinusoidal endothelial cells by immunofluorescence localization.
Conclusion : LratCre strain as a spcific reporter mouse for hepatic stellate cells can be uesd to trace hepatic stellate cells.
- Full text:2024100811311465073利用Cre-Loxp技术构建标记肝星状细胞的小鼠模型_黄紫薇.pdf
