Reproductive damage of male rats exposed to plateau environment
10.19485/j.cnki.issn2096-5087.2024.08.019
- Author:
BU Zihan
;
ZHOU Hao
;
LI Jiahao
;
ZHANG Bin
;
ZHANG Chunlei
;
CHANG Dehui
- Publication Type:Journal Article
- Keywords:
plateau;
reproductive system;
testicle;
oxidative stress;
sex hormone
- From:
Journal of Preventive Medicine
2024;36(8):727-730,736
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of plateau environment exposure on the reproductive system of male rats, so as to provide the reference for mechanisms of reproductive damage in plateau environment.
Methods:Sixty SPF-grade 12-week-old male Wistar rats were randomly divided into the plain-exposed group, the 1 day-, 3 day-, 7 day-, 14 day- and 28 day- plateau-exposed groups. The rats in the plain-exposed group were raised under normal conditions for 28 days, while the rats in the plateau-exposed groups were raised in a simulated high-altitude plateau chamber. After the completion of the designated feeding periods, the rats were sacrificed under anesthesia, and testicular tissue and abdominal aortic blood were collected to detect the testicular index and evaluate sperm quality. Histological and cellular morphologies of the testicular tissue were analyzed. Additionally, the levels of malondialdehyde (MDA), superoxide dismutase (SOD) and reactive oxygen (ROS) in the testicular tissue were determined, along with serum levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH) and testosterone (T).
Conclusions:Plateau environment may cause a decrease in testicular index and sperm quality, impair mitochondrial function, induce oxidative stress, and thus affect reproductive system of male rats. However, there are signs of self-repair in the reproductive system with the increase of exposure duration.
- Full text:2024100810003170456雄性大鼠高原环境暴露生殖系统损伤研究.pdf
