Effect of Electricity Targeting TGF-β 1/smads Signaling in Oligoasthenthymic Rats
10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).20240907.007
- VernacularTitle:基于TGF-β1/smads信号通路研究电针对少弱精症大鼠的影响
- Author:
Chunxue MENG
1
,
2
;
Fei WANG
;
Wenjing SUN
;
Nan YANG
;
Hailong ZHAO
;
Shengfu ZHANG
;
Huisheng MA
;
Bin GUO
Author Information
1. 宁夏医科大学生育力保持教育部重点实验室,宁夏 银川 750004
2. 宁夏医科大学中医学院,宁夏 银川 750004
- Keywords:
oligoasthenspermia;
acusector;
sperm quality;
sex hormone;
TGF-β 1/smads signaling pathway
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2024;45(5):797-805
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To explore the effects of electricity on sperm quality,sex hormone levels,and related proteins in TGF-β 1/smads signaling in testicular tissue.[Methods]Forty male SD rats were randomly divided into blank group,model group,electroacupuncture group,and positive drug group.Adenine gavage method was used to establish the model,in the electroacupuncture group,rats received electroacupuncture treatment at the acupoints Zhongji,Guanyuan,Zusanli,and Sanyinjiao once daily for 30 minutes;in the positive drug group,rats were administered L-carnitine oral solution via gavage at a dosage of 10 mL/kg,both intervention groups underwent continuous treatment for 28 days.After treatment,the changes of sperm number,sperm motility rate,follicle-stimulating hormone(FSH),luteizing hormone(LH)and serum sex hormone testosterone(T)were detected by HE staining,and the expression of TGF-β-1/smads signaling pathway in testicular tissue was examined by Western Blot.[Results]Compared to the blank group,the model group showed a significant decrease in sperm count and sperm motility rate(P<0.05),both the electroacupuncture group and the positive drug group exhibited significant increases compared to the model group(P<0.05).Compared to the blank group,the model group had a significant decrease in serum testosterone(T)levels(P<0.05),and significant increases in serum FSH and LH levels(P<0.05);in comparison to the model group,the electroacupuncture group and the positive drug group showed significant increases in serum T levels(P<0.05),and significant decreases in serum FSH and LH levels(P<0.05).The HE staining results showed typical pathological features of AR in the testicular tissue of model group rats,with varying degrees of improvement observed in electroacupuncture and positive drug group rats.Western Blot analysis revealed that in the model group,the protein levels of TGF-β,p-smad2,and p-smad3 in the TGF-β1/smads pathway in testicular tissue were significantly increased(P<0.05);in comparison to the model group,the electroacupuncture group and the positive drug group showed significant decreases in the protein levels of TGF-β,p-smad2,and p-smad3(P<0.05).[Conclusion]Acupuncture can improve the sperm number and sperm motility rate,regulate sex hormone level and improve the spermatogenic environment,and affect the spermatogenesis process via TGF-β 1/smads signaling pathway.
