Application of HIV nucleic acid test in detecting western blot indeterminate and negative specimens
10.3969/j.issn.1006-2483.2024.05.025
- VernacularTitle:HIV核酸补充试验在检测WB不确定及阴性样本中的应用
- Author:
Cong LIU
1
;
Meng GUO
1
;
Fanghua MEI
1
;
Kun CAI
1
;
Yake LEI
1
Author Information
1. Hubei Provincial Center for Disease Control and Prevention , Wuhan , Hubei 430079 , China
- Publication Type:Journal Article
- Keywords:
HIV;
Western blot indeterminate/negative;
HIV nucleic acid test
- From:
Journal of Public Health and Preventive Medicine
2024;35(5):105-108
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the application of nucleic acid test in the diagnosis of HIV western blotting (WB) indeterminate and negative samples. Methods A total of 2 518 HIV samples to be confirmed were collected from the Hubei HIV Confirmation Center Laboratory from 2014 to 2022. The results of follow-up antibody test and nucleic acid test of WB indeterminate and negative samples were analyzed, and the diagnostic rate, sensitivity, specificity and coincidence rate of the two detection strategies were compared. Results There were totally 133 indeterminate or negative samples by WB test. Of the 99 indeterminate samples , 76 (76.77%) had nucleic acid test results >5000 copies/mL. 40 cases were followed up, of which 33 cases (82.50%) turned positive and 7 (17.50%) were still uncertain. Of the 7 samples with 20~5000 copies /mL (7.07%), 2 cases were followed up and both turned positive during the follow-up. 1 case (1.01%) with nucleic acid <20 copies/mL turned positive during follow-up. Among the 34 WB negative samples, 15 cases (45.45%) were > 5 000 copies/mL, 9 of which were seroconversion. The diagnostic rates of “antibody confirmation” and “nucleic acid test” were 96.78% (2 437/2 518) and 99.60% (2 508/2 518), respectively. The sensitivities were 98.02% (2 430/2 479) and 99.88% (2476/2 479), and the specificities were 78.13% (25/32) and 100.00 % (32/32), respectively. The coincidence rates were 97.77% (2 455/2 511) and 99.88% (2 508/2 511), respectively. Conclusion Nucleic acid supplemental test strategy has high diagnostic rate, sensitivity, specificity and coincidence rate. WB indeterminate and negative samples can be diagnosed as early as possible through nucleic acid supplemental testing.
