Identification and test methods of rare unexpected antibodies in grassroots medical institutions
10.13303/j.cjbt.issn.1004549x.2024.09.019
- VernacularTitle:基层医院对罕见意外抗体的鉴定思路与试验方法探讨
- Author:
Xun LI
1
;
Zhixian SONG
1
;
Hongmei AI
1
;
Junli LI
1
Author Information
1. Department of Blood Transfusion, Jingzhou Central Hospital/Research Institute of Blood Transfusion, Yangtze University, Jingzhou 434020, China
- Publication Type:Journal Article
- Keywords:
unexpected antibody;
pedigree study;
high-prevalence antigen series;
irradiation
- From:
Chinese Journal of Blood Transfusion
2024;37(9):1073-1076
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To explore how to identify rare unexpected antibodies using routine serology methods in blood transfusion department of grassroots hospitals. 【Methods】 A patient with gastrointestinal bleeding with inconsistent forward and reverse blood typing, positive antibody screening and incompatible blood matching in our hospital was selected as the research object. Mixed human O cell absorption method was used for blood type identification, and 10-panel reagent red blood cells, manual polybrene test, 2-mercaptoethanol(2-ME) test, blind match and pedigree study were used to explore the antibody characteristics. 【Results】 The serological test results of the patient′s serum were as follows: The blood group was type B and RhD positive, and negative for direct antiglobulin test; The antibody was IgM; The likelihood of this antibody being anti-s, anti-Fya or anti-k was low, and compound antibodies could not be excluded; The blood of the patient did not match with blood samples of 20 blood donors in the major side, while it matched with all the blood samples of her three sisters. 【Conclusion】 When the antibodies of high-prevalence antigen series in plasma cannot be identified, the matched blood may be found tthrough family survey for irradiation and transfusion.
