Serum Level of G-CSF and GM-CSF and CFU-GM Response to Hematopoietic Growth Factors in a Patient with Cyclic Neutropenia.
- Author:
Youn Kyung LEE
1
;
Seok Hun KIM
;
Kwang Woo KIM
;
Soon LEE
;
Heung Sik KIM
;
Dong Seok CHUN
;
Kun Soo LEE
Author Information
1. Department of Pediatrics, Sunlin Hospital, Handong University, Pohang, Korea.
- Publication Type:Original Article
- Keywords:
Cyclic neutropenia;
G-CSF;
GM-CSF;
SCF;
CFU-GM;
CD34
- MeSH:
Bone Marrow;
Child;
Enzyme-Linked Immunosorbent Assay;
Flow Cytometry;
Granulocyte Colony-Stimulating Factor*;
Granulocyte-Macrophage Colony-Stimulating Factor*;
Granulocyte-Macrophage Progenitor Cells*;
Hematopoiesis;
Humans;
Intercellular Signaling Peptides and Proteins*;
Male;
Methylcellulose;
Neutropenia*;
Neutrophils;
Stem Cells
- From:Journal of the Korean Pediatric Society
2001;44(10):1168-1175
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Serum levels of G-CSF and GM-CSF were measured and CFU-GM assay using G- CSF, GM-CSF and SCF was conducted to evaluate the influence of hematopoietic growth factor on the precursor cells of cyclic neutropenia. METHODS: A 7-year-old male with cyclic neutropenia was studied. Marrow mononuclear cells were isolated at neutrophil nadir and recovery and cultured in methylcellulose media with or without G-CSF, GM- CSF and SCF. CD34 positive cells were evaluated using flow cytometry. Serum levels of G-CSF and GM-CSF were measured by ELISA. RESULTS: The Numbers of CFU-GM without growth factors were 50 at neutrophil nadir and 33 at the recovery phase in the patient and show increased colony forming capacity. CD34 positive cells were 9.32% at nadir and 14.17% at recovery. Increasement of CFU-GM with G-CSF at nadir and recovery were 46% and 118% and those with GM-CSF were 70% and 78% respectively, compared with 54.4% and 78.2% in control groups. In contrast, the presence of SCF did not enhance CFU-GM number in the patient, but in the control group, increasement with SCF was 28.9 %. There an was inverse relationship between serum G-CSF levels and peripheral neutrophil count whereas those of GM-CSF were constant. CONCLUSION: Serum G-CSF level showed inverse relationship with neutrophil counts. The response of progenitor cells to G-CSF and GM-CSF was not impaired. The presence of SCF did not enhance CFU-GM number in the patient. This result suggests that the abnormality in hematopoiesis in cyclic neutropenia may involve more immature progenitor cells responsive to SCF.
