Expressions and clinical significance of UBE2I and FCGR1A in AIDS complicated with active pulmonary tuberculosis
- VernacularTitle:UBE2I和FCGR1A在艾滋病合并活动性肺结核患者中的表达及临床意义
- Author:
Min WANG
1
;
Wenqin YANG
1
;
Mengrui SHI
1
;
Rongqiang ZHANG
1
;
Xiaoli WANG
2
;
Zhigang ZHANG
1
Author Information
- Publication Type:Journal Article
- Keywords: acquired immune deficiency syndrome (AIDS); active pulmonary tuberculosis (APTB); UBE2I; FCGR1A; valproic acid
- From: Journal of Xi'an Jiaotong University(Medical Sciences) 2024;45(5):782-788
- CountryChina
- Language:Chinese
- Abstract: 【Objective】 To explore the effect of UBE2I and FCGR1A gene expressions on the incidence of acquired immune deficiency syndrome (AIDS) combined with active pulmonary tuberculosis (APTB), so as to provide basis for disease monitoring. 【Methods】 A total of 98 AIDS patients combined with APTB and 84 AIDS patients combined with latent tuberculosis infection (LTBI) were selected from the validated whole genome transcriptome dataset (GSE37250). The top 30 differentially expressed genes (DEGs) in the two groups of patients were screened. We established the PPI interaction network, transcription factor-differentially expressed gene (TF-DEG), DEG-miRNA, and environmental chemical regulation network of the top 30 DEGs. Receiver operating characteristic (ROC) curves of 11 key DEGs were plotted and Logistic regression analysis was performed. 【Results】 There were 6 054 DEGs in the two groups of patients, and UBE2I was an important core node of the PPI interaction network. FCGR1A had the best predictive and indicative ability for AIDS combined with APTB. Univariate Logistic regression showed that high expressions of UBE2I and FCGR1A were risk factors for AIDS combined with APTB (P<0.05). The regulatory network showed that VEGFB was a key gene in the TF-DEG network, participating in regulation with transcription factors such as SEPT9 and SMAD5. It targeted miRNAs such as hsa-mir-17-5p and hsa-mir-20a-5p, and was affected by environmental chemicals such as valproic acid and copper sulfate. 【Conclusion】 VEGFB plays an important role in the pathogenesis of AIDS combined with APTB. The abnormally high expressions of UBE2I and FCGR1A are associated with the disease progression of AIDS combined with APTB. The disease condition can be monitored by detecting the expression level of UBE2I and FCGR1A.
