Correlation of changes in serum T lymphocyte subsets levels with disease severity and prognosis in patients with ankylosing spondylitis
- VernacularTitle:强直性脊柱炎患者T淋巴细胞亚群水平变化与疾病严重程度及预后的相关性分析
- Author:
Lizhen CHEN
1
;
Jinxing SHI
1
;
Xiaolin LIU
1
;
Shengjie XU
1
;
Wenping LIN
2
Author Information
- Publication Type:Journal Article
- Keywords: ankylosing spondylitis; T lymphocyte subset; severity of disease; prognosis
- From: Journal of Xi'an Jiaotong University(Medical Sciences) 2024;45(5):769-775
- CountryChina
- Language:Chinese
- Abstract: 【Objective】 To study the changes in peripheral blood T lymphocytes in patients with ankylosing spondylitis and their correlation with the disease’s severity and prognosis. 【Methods】 We selected 120 patients with ankylosing spondylitis treated between January 2020 and March 2023 as the research group and 120 healthy people who had medical examinations in the same period as the health group. We detected the changes in CD4+, CD8+ and CD4+/CD8+ values of peripheral blood T lymphocyte subsets with flow cytometry, compared the differences in T lymphocyte subsets between the two groups, and analyzed the correlation with the disease severity of the patients. All the 120 patients with ankylosing spondylitis were followed up for 6 months after treatment to assess their prognosis. General information and T lymphocyte sub-groups CD4+, CD8+ level, CD4+/CD8+ value changes were compared among patients with different prognosis. We analyzed the value of T lymphocyte sub-groups in predicting the prognosis of patients with ankylosing spondylitis. 【Results】 In the research group CD4+ and CD4+/CD8+ were lower but CD8+ was higher than those in the healthy group (P<0.05). CD4+ and CD4+/CD8+ were lower but CD8+ was higher in patients with advanced ankylosing spondylitis than in early and mid-term patients (P<0.05). The ROC curve analysis showed that the AUC of CD4+, CD8+, and CD4+/CD8+ combined diagnosis of ankylosing spondylitis patients was 0.878, with higher diagnostic sensitivity than that of the single diagnosis (P<0.05). In the poor prognosis group, CD8+ was higher than that in the excellent prognosis group, but CD4+ and CD4+/CD8+ value were lower than the latter (P<0.05). The results of Pearson test showed that CD4+ and CD4+/CD8+ were negatively correlated with the prognosis of patients with ankylosing spondylitis (r=-0.568, -0.656, P<0.001). CD8+ was positively correlated with the prognosis of patients with ankylosing spondylitis (r=0.623, P<0.001). ROC curve analysis showed that the AUC of the combined diagnosis of CD4+, CD8+ and CD4+/CD8+ for ankylosing spondylitis patients was 0.910, and the diagnostic sensitivity was higher than that of single diagnosis (P<0.05). 【Conclusion】 The abnormal levels of peripheral blood T lymphocyte subsets in patients with ankylosing spondylitis are closely related to the severity and prognosis of the disease, and can be used as a reference indicator for diagnosing the severity and prognosis of ankylosing spondylitis.
