SARS-CoV-2 induces cardiomyocyte apoptosis by targeting the CypA/CD147 receptor pathway
- VernacularTitle:SARS-CoV-2靶向CypA/CD147受体途径诱导心肌细胞凋亡
- Author:
Mingren MA
1
;
Ling MA
;
Yan LIU
;
Fei WANG
Author Information
- Keywords: severe acute respiratory syndrome coronavirus 2(SARS-CoV-2); coronavirus disease 2019(COVID-19); cyclophilin A(CypA)/CD147; cardiomyocyte; signaling pathway; apoptosis
- From: Journal of Xi'an Jiaotong University(Medical Sciences) 2024;45(5):734-740
- CountryChina
- Language:Chinese
- Abstract: Objective To explore the possible mechanism of apoptosis induced by SARS-CoV-2 targeting cyclophilin A(CypA)/CD147 receptor pathway.Methods The pEncmv-SARS-CoV-2 S-3xflag recombinant plasmid was constructed and transfected into H9C2 cells.Apoptosis was analyzed by TUNEL assay,flow cytometry and DNA ladder.The interaction between CD147 and SARS-CoV-2 S protein was verified by CoIP.The expressions of apoptosis-related proteins were detected by Western blotting.Results Compared with the control group,TUNEL assay and flow cytometry showed that apoptosis was significantly increased in transfection group(P＜0.05),and DNA ladder analysis showed that DNA degradation was obvious.The expression levels of Caspase12 and DR3 were significantly increased(P＜0.001),the expression level of FAS was slightly increased(P＜0.01),while the expression levels of TRF1 and DR4 were decreased(P＜0.01).Conclusion SARS-CoV-2 S protein targets CypA/CD147 receptor to invade host cells,activate endoplasmic network pathway(Caspase12)and death receptor pathway(DR3,FAS),and induce apoptosis of cardiomyocytes through endogenous and exogenous apoptotic pathways.
