SARS-CoV-2 induces cardiomyocyte apoptosis by targeting the CypA/CD147 receptor pathway
- VernacularTitle:SARS-CoV-2靶向CypA/CD147受体途径诱导心肌细胞凋亡
- Author:
Mingren MA
1
;
Ling MA
1
;
Yan LIU
1
;
Fei WANG
1
Author Information
- Publication Type:Journal Article
- Keywords: severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); coronavirus disease 2019 (COVID-19); cyclophilin A (CypA)/CD147; cardiomyocyte; signaling pathway; apoptosis
- From: Journal of Xi'an Jiaotong University(Medical Sciences) 2024;45(5):734-740
- CountryChina
- Language:Chinese
- Abstract: 【Objective】 To explore the possible mechanism of apoptosis induced by SARS-CoV-2 targeting cyclophilin A (CypA)/CD147 receptor pathway. 【Methods】 The pEncmv-SARS-CoV-2 S-3xflag recombinant plasmid was constructed and transfected into H9C2 cells. Apoptosis was analyzed by TUNEL assay, flow cytometry and DNA ladder. The interaction between CD147 and SARS-CoV-2 S protein was verified by CoIP. The expressions of apoptosis-related proteins were detected by Western blotting. 【Results】 Compared with the control group, TUNEL assay and flow cytometry showed that apoptosis was significantly increased in transfection group (P<0.05), and DNA ladder analysis showed that DNA degradation was obvious. The expression levels of Caspase12 and DR3 were significantly increased (P<0.001), the expression level of FAS was slightly increased (P<0.01), while the expression levels of TRF1 and DR4 were decreased (P<0.01). 【Conclusion】 SARS-CoV-2 S protein targets CypA/CD147 receptor to invade host cells, activate endoplasmic network pathway (Caspase12) and death receptor pathway (DR3, FAS), and induce apoptosis of cardiomyocytes through endogenous and exogenous apoptotic pathways.
