Blood metabolomics research for calcium oxalate urolithiasis in adults based on UPLC-Q-TOF/MS
10.3969/j.issn.1009-8291.2024.09.015
- VernacularTitle:基于UPLC-Q-TOF/MS技术的成人草酸钙尿石症血液代谢组学研究
- Author:
Xiangyu WANG
1
;
Jiayuan JI
1
;
Teng CUI
1
;
Jing XIAO
1
Author Information
1. Department of Urology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China
- Publication Type:Journal Article
- Keywords:
biomarker;
metabolomics;
ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS);
calcium oxalate;
urolithiasis;
blood metabolomics
- From:
Journal of Modern Urology
2024;29(9):823-829
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 Based on ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS), the potential biomarkers and metabolic mechanisms of adult calcium oxalate urolithiasis were identified to guide the diagnosis and treatment of calcium oxalate lithiasis. 【Methods】 Blood samples of 36 patients diagnosed with bilateral upper urinary tract calcium oxalate stones and 36 healthy controls admitted to our hospital during Oct.2017 and Jul.2018 were collected.Metabolic fingerprints were analyzed with UPLC-Q-TOF/MS.The multivariate data were analyzed with principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA).Potential biological markers were identified by comparing human metabolome datasets.Finally, the metabolic pathways of the identified compounds were analyzed and the metabolic network map was constructed. 【Results】 PCA and OPLS-DA showed significant differences in blood metabolites between patients and healthy controls, and identified 29 blood-related metabolites, including linoleic acid, citric acid, uric acid and so on.The most relevant blood metabolic pathways were bile acid synthesis pathway, fatty acid biosynthesis pathway, glycerophospholipid metabolism pathway, sphingolipid metabolism pathway and purine metabolism pathway. 【Conclusion】 Blood metabolomics analysis based on the UPLC-Q-TOF/MS platform identified 29 potential blood biomarkers of adult calcium oxalate urolithiasis, and purine and lipid metabolism may be related to the genesis of calcium oxalate stones.
