Effects and mechanism of β-asarone on inhibiting cell proliferation/migration/invasion of renal cell carcinoma
10.3969/j.issn.1009-8291.2024.08.015
- VernacularTitle:β-细辛醚对肾癌增殖及迁移侵袭的影响及其分子机制
- Author:
Pan ZHANG
1
;
Yibo MEI
;
Dalin HE
;
Jin ZENG
Author Information
1. 西安交通大学第一临床医学院,陕西西安 710061
- Keywords:
renal cell carcinoma;
β-asarone;
autophagy;
migration;
invasion;
epithelial-mesenchymal transition;
AMPK;
mTOR
- From:
Journal of Modern Urology
2024;29(8):731-736
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects and molecular mechanism of β-asarone(β-As)on renal cell carcinoma(RCC).Methods Human RCC 786-O and 769-P cells were used in in vitro experiments,and murine RCC Renca cells were used in in vivo experiments.MTT,wound healing assay and Transwell assay were used to evaluate cell migration and invasion.Western blot was used to detect changes in protein levels during autophagy and epithelial-mesenchymal transition(EMT).Xenograft models of Balb/c mice were used to detect the anti-tumor effect of β-As in vivo.Results β-As inhibited cell growth in a concentration-dependent manner;upregulated the expression of E-cadherin,but down-regulated the expressions of N-cadherin and Vimentin;inhibited cell proliferation,migration and invasion.β-As upregulated the expression of LC3 and p-AMPK,downregulated the expressions of p-mTOR and p-S6K in a concentration-dependent manner,but had no effects on the expressions of AMPK and mTOR.β-As inhibited the proliferation of RCC cells in vivo.Conclusion β-As inhibits the proliferation,migration,invasion and EMT of RCC cells through the autophagy-dependent AMPK/mTOR signaling pathway,which may provide new ideas for the treatment of RCC,especially advanced RCC.
