Effects of lncRNA SNHG12 on the proliferation, migration and invasion of prostate cancer cells by targeting miR-495-3p/PI3K/Akt signaling pathway
10.3969/j.issn.1009-8291.2024.07.016
- VernacularTitle:lncRNA SNHG12靶向抑制miR-495-3p进而调控PI3K/Akt信号通路对前列腺癌细胞增殖、迁移和侵袭的影响
- Author:
Li TIAN
1
;
Haijun CUI
2
;
Jinheng XU
1
;
Yueming HU
1
;
Jihua ZHAO
3
;
Bohai CAO
1
Author Information
1. Department of Pathology, Tangshan Central Hospital, Tangshan 063000
2. Department of Urology, Tangshan Central Hospital, Tangshan 063000
3. Clinical Medicine School of North China University of Technology, Tangshan 063000, China
- Publication Type:Journal Article
- Keywords:
lncRNA;
SNHG12;
miR-495-3p;
PI3K/Akt signaling pathway;
prostate cancer;
proliferation;
migration;
invasion
- From:
Journal of Modern Urology
2024;29(7):642-648
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To explore the effects of long non-coding RNA (lncRNA) small nucleolar molecule RNA host gene 12 (SNHG12) targeting inhibition of miR-495-3p/ phospholipinositol-3-kinase (PI3K)/protein kinase B (Akt) signaling pathway on the proliferation, migration and invasion of prostate cancer cells. 【Methods】 The expressions of SNHG12 and miR-495-3p in prostate cancer tissues and cells (LNCaP, C4-2, DU145) were detected with real-time fluorescence quantitative PCR (qRT-PCR).After DU145 cells were divided into si-NC, si-SNHG12, si-SNHG12+anti-miR-NC and si-SNHG12+anti-miR-495-3p groups, the expressions of SNHG12 and miR-495-3p were detected with qRT-PCR; the targeting relationship between SNHG12 and miR-495-3p was determined with dual luciferase assay; cell proliferation was assessed with MTT assay; cell migration and invasion were evaluated with Transwell assay; the protein expressions of proliferating cell nuclear antigen (PCNA), N-cadherin, and E-cadherin were detected with Western blot. 【Results】 The expressions of SNHG12 were significantly increased, while the expression of miR-495-3P was significantly decreased in prostate cancer tissues and cells (LNCaP, C4-2, DU145) (P<0.05).Knockdown of SNHG12 decreased DU145 cell activity, lowered the protein expressions of PCNA and N-cadherin, reduced the number of migrating and invading cells, but increased the protein expression of E-cadherin (P<0.05).SNHG12 targeted and negatively regulated miR-495-3p, and down-regulation of miR-495-3p reversed the effects of SNHG12 knockdown on the proliferation, migration and invasion of prostate cancer cells.Compared with the si-NC group, the si-SNHG12 group had significantly decreased expressions of p-PI3K and p-Akt (P<0.05).Compared with the si-SNHG12+anti-miR-NC group, the si-SNHG12+anti-miR-495-3p group had significantly increased protein expressions of p-PI3K and p-Akt (P<0.05). 【Conclusion】 lncRNA SNHG12 can promote the proliferation, migration and invasion of prostate cancer cells through targeted inhibition of miR-495-3p/PI3K/Akt signaling pathway.
