Lipopolysaccharide regulates neutrophil inflammation through activating the LRG1/ROCK1 signaling

Qiao Feng; Xin Han; Bohui Yuan; Xuejiao Zhang; Hui Hua; Wanpeng Cheng; Suping Qin; Feng Zhou; Xiaomei Liu

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Lipopolysaccharide regulates neutrophil inflammation through activating the LRG1/ROCK1 signaling

VernacularTitle:脂多糖激活LRG1/ROCK1信号轴调控中性粒细胞炎症反应
Author: Qiao FENG ¹ ; Xin HAN ¹ ; Bohui YUAN ¹ ; Xuejiao ZHANG ¹ ; Hui HUA ¹ ; Wanpeng CHENG ¹ ; Suping QIN ¹ ; Feng ZHOU ¹ ; Xiaomei LIU ¹
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1. Jiangsu Key Laboratory of Immunity and Metabolism, Xuzhou Medical University, Xuzhou 221004, China
Publication Type:Journal Article
Keywords: lipopolysaccharide (LPS); neutrophil; LRG1; ROCK1; inflammatory response
From: Journal of Xi'an Jiaotong University(Medical Sciences) 2024;45(4):597-602
CountryChina
Language:Chinese
Abstract: 【Objective】 To investigate the role of lipopolysaccharide (LPS) in regulating the inflammatory response of neutrophil through the leucine-rich α-2 glycoprotein 1 (LRG1)/ Rho-associated protein kinase (ROCK1) signaling. 【Methods】 HL-60 cells were treated with 1 μmol/L all-trans retinoic acid (ATRA) and 12.5 μL/mL dimethyl sulfoxide (DMSO) for 72 h and 96 h, and the morphological changes were observed by Wright-Giemsa staining. The expression of CD11b was detected by flow cytometry. LPS induced the activation of dHL-60 and human peripheral blood neutrophils. The transcription and secretion levels of LRG1, ROCK1 and inflammatory cytokines were detected by qPCR and ELISA, respectively. The expression levels of LRG1 and ROCK1 after the activation of dHL-60 were detected by Western blotting. Furthermore, dHL-60 was treated with the recombinant protein LRG1 and ROCK1 inhibitor Y-27632; the transcription levels of inflammatory cytokines were detected by qPCR. 【Results】 Neutrophils were activated by LPS. The expression levels of LRG1 and ROCK1 were significantly increased, and the transcription levels of inflammatory cytokines were significantly increased. The recombinant protein LRG1 activated dHL-60 in vitro, and the transcription levels of ROCK1 and inflammatory cytokines were significantly increased. Using the ROCK1 inhibitor Y-27632, the production levels of inflammatory cytokines were significantly reduced. 【Conclusion】 LPS can regulate the production levels of neutrophil inflammatory cytokines through activating the LRG1/ROCK1 signaling, thus exacerbating the inflammatory response.