Influencing Factors on Degree of Inflammation in Experimental Autoimmune Uveitis Rat Model and Characteristics of Traditional Chinese and Western Medicine Symptoms
10.13422/j.cnki.syfjx.20241311
- VernacularTitle:实验性自身免疫葡萄膜炎大鼠模型炎症程度影响因素及中西医病证特点分析
- Author:
Liang LIU
1
;
Xiaoyu LI
2
;
Xiaofeng HAO
2
;
Hang YUAN
2
;
Yige ZHANG
2
;
Like XIE
2
Author Information
1. Tianjin University of Traditional Chinese Medicine， Tianjin 301617， China
2. Ophthalmology Hospital of the China Academy of Chinese Medical Sciences， Beijing 100040， China
- Publication Type:Journal Article
- Keywords:
experimental autoimmune uveitis;
factors influencing modeling;
integration of traditional Chinese and Western medicine;
disease and symptom characteristics
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2024;30(21):104-111
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the effects of different emulsion mixtures and emulsification methods on the inflammation severity in an experimental autoimmune uveitis （EAU） model in rats， and to analyze the characteristics of the current EAU model. MethodEAU was induced in Lewis rats by subcutaneous injection of interphotoreceptor retinoid-binding protein （IRBP） 1177-1191 emulsified with Freund's complete adjuvant （CFA）， with or without intraperitoneal injection of pertussis toxin （PTX）. Slit lamp examination， HE staining， and optical coherence tomography were used to evaluate factors affecting EAU modeling， including different doses of the emulsion mixture （IRBP1177-1191， PTX， and inactivated Mycobacterium tuberculosis） and four different emulsification methods. The classification， characteristics， modeling methods， advantages， and disadvantages of EAU animal models were summarized and analyzed based on the clinical diagnostic criteria and syndrome characteristics of chronic uveitis in both traditional Chinese medicine （TCM） and western medicine， to evaluate the consistency between TCM and western medical syndromes. ResultIncreasing the dose of inactivated M. tuberculosis and antigen peptide in the emulsion mixture exacerbated the anterior segment inflammation in EAU rats. Increasing the injection of PTX also exacerbated anterior segment inflammation and increased retinal thickness in EAU rats. The severity of the EAU model was closely related to the emulsification method used. All four emulsification methods successfully induced EAU in rats. Comparatively， the ultrasonic cell disruptor and T10 basic disperser achieved successful emulsification in a short time. The degree of emulsification of the mixture also influenced the severity of the EAU model in rats. The existing EAU animal model shows a high degree of consistency with western medical diagnoses and the main ocular syndromes in TCM. ConclusionIRBP1177-1191， PTX， inactivated M. tuberculosis， and emulsification methods can affect the severity of the EAU model through different pathways. The existing EAU animal models can simulate the clinical characteristics of western medicine well but lack the etiology， pathogenesis， and syndrome characteristics of TCM. Therefore， it is necessary to construct an EAU animal model that combines disease and syndrome characteristics.
