Analysis of p53 tumor suppressor gene mutations and human papillomavirus infection in human bladder cancers.
10.3349/ymj.1995.36.4.322
- Author:
Kun Hong KIM
1
;
Yoon Soo KIM
Author Information
1. Department of Biochemistry and Molecular Biology, Yonsei University College of Medicine, Seoul, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
p53 tumor suppressor gene;
polymerase chain reaction;
single strand conformation polymorphism analysis;
human papillomavirus;
transitional cell carcinoma of bladder
- MeSH:
Base Sequence;
Bladder Neoplasms/*genetics/pathology/*virology;
Dyes;
Genes, Tumor Suppressor;
*Genes, p53;
Human;
Immunohistochemistry/methods;
Molecular Probes/genetics;
Molecular Sequence Data;
*Mutation;
*Papillomavirus, Human/classification/isolation & purification;
Papovaviridae Infections/*complications;
Polymorphism, Single-Stranded Conformational;
Support, Non-U.S. Gov't;
Tumor Virus Infections/*complications
- From:Yonsei Medical Journal
1995;36(4):322-331
- CountryRepublic of Korea
- Language:English
-
Abstract:
To determine whether the dysfunction of p53 caused either by mutation of the p53 gene itself or by binding to E6 protein of oncogenic HPVs is involved in the transitional cell carcinomas (TCCs) of the bladder, we analyzed 23 TCCs of the bladder. DNA was extracted from each paraffin embedded tissue of TCCs of bladder and polymerase chain reaction (PCR)/single strand conformation polymorphism (SSCP) analysis were performed to screen mutations in p53 tumor suppressor gene, then PCR/dot blot hybridization were performed to detect infection of HPVs. We found that p53 gene mutation was found in 3 cases and oncogenic HPV infection was detected in 8 cases and thus, the overall incidence of possible p53 dysfunction was 47.8% on DNA analysis (If the results of immunohistochemistry to detect overexpression of p53 protein were included, the incidence was 60.9%). Therefore, we concluded that dysfunction of p53 plays a major role in the development of TCCs of bladder in Korean patients.
