Identification of chemical constituents of modified Yupingfengsan formula
- VernacularTitle:玉屏风散加味方化学成分鉴定
- Author:
Cuizhen LIU
1
;
Siqi FENG
1
;
Xiujuan LIN
1
;
Qin LU
1
;
Yusheng HUANG
2
,
3
;
Lijuan ZHANG
3
,
4
;
Detang LI
3
Author Information
1. School of First Clinical Medicine，Guangzhou University of Chinese Medicine，Guangzhou 510405，China
2. Lingnan Medical Research Center，Guangzhou University of Chinese Medicine，Guangzhou 510407，China
3. Guangdong Clinical Research Academy of Chinese Medicine，Guangzhou 510405，China
4. Dept. of Otorhinolaryngology，the First Affiliated Hospital of Guangzhou University of Chinese Medicine，Guangzhou 510405，China
- Publication Type:Journal Article
- Keywords:
modified Yupingfengsan formula;
chemical constituents;
flavonoids;
phenylpropanoids;
fragmentation pattern
- From:
China Pharmacy
2024;35(18):2225-2231
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To identify the chemical constituents of the modified Yupingfengsan formula. METHODS UPLC-Q- Exactive Orbitrap-MS technology was adopted. The separation was performed on Waters BEH C18 column with acetonitrile （A）-0.1% formic acid solution （B） as mobile phase for gradient elution. The heating electrospray ionization was used for positive and negative ion mode scanning. The scanning range was m/z 50-1 500， and the spray voltage was 2 kV （positive ion mode） and 1.5 kV （negative ion mode）. The information of chemical constituents of modified Yupingfengsan formula was collected through literature review to establish a database； the structure of the constituent was identified based on the above database， relevant literature， and chromatography and mass spectrometry information of reference standards. RESULTS & CONCLUSIONS Totally 114 chemical constituents were identified from modified Yupingfengsan formula， including 31 flavonoids， 39 phenylpropanoids， 5 saponins， 8 terpenoids， 3 chromones， 3 curcuminoids， etc. Based on the comparison of reference standards， 8 constituents were ultimately determined， including magnoflorine， calycosin， calycosin glycoside， cimifugin， 5-O-methylvisammioside， sec-O- glucosylhamaudol， luteolin and mangiferin. These constituents mainly involved glycosylation cleavage， retro Diels-Alder fragmentation， glycosylation loss， neutral molecule loss and other fragmentation pathways.
