High-plasticity mineral trioxide aggregate and its effects on M1 and M2macrophage viability and adherence, phagocyte activity, production of reactive oxygen species, and cytokines
- Author:
Betânia Canal VASCONCELLOS
1
;
Layara Cristine Tomaz TAVARES
;
Danilo Couto da SILVA
;
Francielen Oliveira FONSECA
;
Francine BENETTI
;
Antônio Paulino Ribeiro SOBRINHO
;
Warley Luciano Fonseca TAVARES
Author Information
- Publication Type:Research Article
- From:Restorative Dentistry & Endodontics 2023;48(1):e6-
- CountryRepublic of Korea
- Language:English
-
Abstract:
Objectives:This study evaluated the effects of high-plasticity mineral trioxide aggregate (MTA-HP) on the activity of M1 and M2 macrophages, compared to white MTA (Angelus).
Materials and Methods:Peritoneal inflammatory M1 (from C57BL/6 mice) and M2 (from BALB/c mice) macrophages were cultured in the presence of the tested materials. Cell viability (MTT and trypan blue assays), adhesion, phagocytosis, reactive oxygen species (ROS) production, and tumor necrosis factor (TNF)-α and transforming growth factor (TGF)-β production were evaluated. Parametric analysis of variance and the non-parametric Kruskal-Wallis test were used. Results were considered significant when p < 0.05.
Results:The MTT assay revealed a significant decrease in M1 metabolism with MTA-HP at 24 hours, and with MTA and MTA-HP later. The trypan blue assay showed significantly fewer live M1 at 48 hours and live M2 at 48 and 72 hours with MTA-HP, compared to MTA. M1 and M2 adherence and phagocytosis showed no significant differences compared to control for both materials. Zymosan A stimulated ROS production by macrophages. In the absence of interferon-γ, TNF-α production by M1 did not significantly differ between groups. For M2, both materials showed higher TNF-α production in the presence of the stimulus, but without significant between-group differences. Likewise, TGF-β production by M1 and M2 macrophages was not significantly different between the groups.
Conclusions:M1 and M2 macrophages presented different viability in response to MTA and MTA-HP at different time points. Introducing a plasticizer into the MTA vehicle did not interfere with the activity of M1 and M2 macrophages.
