Adenosine Deaminase in Human Skin.
- Author:
Young Pio KIM
;
Johng Bong KAHNG
;
Joon Yul CHON
;
Chull Wan IHM
- Publication Type:Original Article
- MeSH:
Adenosine Deaminase*;
Adenosine*;
Centrifugation;
Deamination;
Dermis;
Epidermis;
Foreskin;
Humans*;
Hydrogen-Ion Concentration;
Inosine;
Skin*
- From:Korean Journal of Dermatology
1981;19(4):385-389
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Adenosine deaminase (adenosine aminohydrolase, AL)A), which catalyzes the deamination of adenosine to yield inosine and amrnonia, was assayed in human penile foreskin. The skin tissue was separated into two layers; epidermis and dermis. They were sliced with scissors into gel state, 4 volumes of 0.05M phosphate buffer solution were added and the tissue homogenized. After centrifugation at 4,000xg for 5 minutes, the supernatant was used as an enzyme solution. ADA activity was measured according to the method f Giuseppe" ADA was found to be present in both layers (epidermis; 0.24 OD/mg protein, dermis; 0.19 OD/mg protein) with slightly higher activity in the epidermis. As in earlier reports, it was found that ADA in the skin showed nearly even activity in the pH range of 5.0-8.0. Considering the significance of ADA in immunological function, the presence of ADA in the skin suggests that the tissue may participate in the immune function.
