Detection of Listeria monocytogenes in CSF from Three Patients with Meningoencephalitis by Next-Generation Sequencing.
10.3988/jcn.2016.12.4.446
- Author:
Ming YAO
1
;
Jiali ZHOU
;
Yicheng ZHU
;
Yinxin ZHANG
;
Xia LV
;
Ruixue SUN
;
Ao SHEN
;
Haitao REN
;
Liying CUI
;
Hongzhi GUAN
;
Honglong WU
Author Information
1. Department of Neurology, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China. guanhz@263.net
- Publication Type:Original Article
- Keywords:
Listeria monocytogenes;
meningoencephalitis;
cerebrospinal fluid;
next generation sequencing
- MeSH:
Central Nervous System;
Cerebrospinal Fluid;
Communicable Diseases;
Diagnosis;
Early Diagnosis;
Encephalitis;
Humans;
Listeria monocytogenes*;
Listeria*;
Magnetic Resonance Imaging;
Meningitis, Listeria;
Meningoencephalitis*;
Methods
- From:Journal of Clinical Neurology
2016;12(4):446-451
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND AND PURPOSE: Encephalitis caused by Listeria monocytogenes (L. monocytogenes) is rare but sometimes fatal. Early diagnosis is difficult using routine cerebrospinal fluid (CSF) tests, while next-generation sequencing (NGS) is increasingly being used for the detection and characterization of pathogens. METHODS: This study set up and applied unbiased NGS to detect L. monocytogenes in CSF collected from three cases of clinically suspected listeria meningoencephalitis. RESULTS: Three cases of patients with acute/subacute meningoencephalitis are reported. Magnetic resonance imaging and blood cultures led to a suspected diagnosis of L. monocytogenes, while the CSF cultures were negative. Unbiased NGS of CSF identified and sequenced reads corresponding to L. monocytogenes in all three cases. CONCLUSIONS: This is the first report highlighting the feasibility of applying NGS of CSF as a diagnostic method for central nervous system (CNS) L. monocytogenes infection. Routine application of this technology in clinical microbiology will significantly improve diagnostic methods for CNS infectious diseases.
