Inhibitory Action of 1,3,5-Trihydroxybenzene on UVB-Induced NADPH Oxidase 4 through AMPK and JNK Signaling Pathways
10.4062/biomolther.2024.054
- Author:
Chaemoon LIM
1
;
Mei Jing PIAO
;
Kyoung Ah KANG
;
Pincha Devage Sameera Madushan FERNANDO
;
Herath Mudiyanselage Udari Lakmini HERATH
;
Dae Whan KIM
;
Joo Mi YI
;
Yung Hyun CHOI
;
Jin Won HYUN
Author Information
1. Department of Orthopedic Surgery, Jeju National University Hospital, College of Medicine, Jeju National University, Jeju 63241, Republic of Korea
- Publication Type:Original Article
- From:Biomolecules & Therapeutics
2024;32(4):499-507
- CountryRepublic of Korea
- Language:EN
-
Abstract:
Specific sensitivity of the skin to ultraviolet B (UVB) rays is one of the mechanisms responsible for widespread skin damage. This study tested whether 1,3,5-trihydroxybenzene (THB), a compound abundant in marine products, might inhibit UVB radiationinduced NADPH oxidase 4 (NOX4) in both human HaCaT keratinocytes and mouse dorsal skin and explore its cytoprotective mechanism. The mechanism of action was determined using western blotting, immunocytochemistry, NADP + /NADPH assay, reactive oxygen species (ROS) detection, and cell viability assay. THB attenuated UVB-induced NOX4 expression both in vitro and in vivo, and suppressed UVB-induced ROS generation via NADP + production, resulting in increased cell viability with decreased apoptosis. THB also reduced the expression of UVB-induced phosphorylated AMP-activated protein kinase (AMPK) and phosphorylated c-Jun N-terminal kinase (JNK). THB suppressed UVB-induced NOX4 expression and ROS generation by inhibiting AMPK and JNK signaling pathways, thereby inhibiting cellular damage. These results showed that THB could be developed as a UV protectant.
