Construction of pancreatic cancer organoids and their sensitivity to chemotherapy drugs
- VernacularTitle:胰腺癌类器官模型的构建及其对化疗药物的敏感性试验
- Author:
Jingyu WANG
1
;
Rong HUANG
;
Yan LU
;
Ziran CHEN
;
Xiaojie ZHANG
;
Hu REN
;
Nan ZHANG
;
Dongbing ZHAO
;
Wei SONG
;
Xingguang ZHANG
Author Information
- Keywords: Pancreatic Neoplasms; Organoids; Drug Therapy,Combination; Sensitivity Tests
- From: Journal of Clinical Hepatology 2024;40(9):1853-1858
- CountryChina
- Language:Chinese
- Abstract: Objective To construct and identify a patient-derived organoid model,and to investigate the sensitivity of chemotherapy drugs using this model.Methods Pancreatic cancer cells were obtained from the surgical specimens of two female patients with a confirmed diagnosis of pancreatic cancer after tumor tissue digestion,and then the cells were inoculated into a culture dish using matrigel for three-dimensional culture.Paraffin sections were prepared for HE staining and immunohistochemical staining and were compared with the parent tumor tissue to determine whether the histopathological features of the tumor in vivo were preserved.The pancreatic cancer organoids were treated with seven chemotherapy drugs at different concentrations;Cell Titer-Glo?3D reagent was used to measure cell viability,and the results of drug sensitivity were analyzed.Results Two patient-derived pancreatic cancer organoids were successfully constructed,and HE staining and immunohistochemical staining showed that the pancreatic cancer organoids had consistent histopathological features with the tumors of the corresponding patient.Both pancreatic cancer organoids were more sensitive to gemcitabine monotherapy and the combination of oxaliplatin+SN38+fluorouracil,and patient 1 was more sensitive than patient 2.There were individual differences in the response to drugs between the organoids from different patients.Conclusion The pancreatic cancer organoid model successfully constructed in this study can reflect the histological classification of parent pancreatic tumors and can be used for in vitro chemotherapy drug sensitivity test,which is expected to provide a reference for clinical medication.
