Studies of cocktail therapy with multiple cytokines for neoplasia or infectious disease of the dog I. cDNA cloning of canine IL-3 and IL-6.
- Author:
Il Seob SHIN
;
Hye Ryon KIM
;
Myung Jin NAM
;
Hwa Young YOUN
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Cytokine;
cDNA;
cloning;
PCR;
IL-3;
IL-6;
dog
- MeSH:
Amino Acid Sequence;
Animals;
Base Sequence;
Cloning, Molecular;
Concanavalin A/pharmacology;
DNA, Complementary/*chemistry;
Dogs/blood/genetics/*immunology;
Interleukin-3/chemistry/*genetics;
Interleukin-6/chemistry/*genetics;
Leukocytes, Mononuclear/chemistry/drug effects;
Molecular Sequence Data;
Open Reading Frames/genetics;
Protein Sorting Signals/genetics;
RNA/blood/genetics;
Reverse Transcriptase Polymerase Chain Reaction/veterinary;
Sequence Homology, Amino Acid;
Sequence Homology, Nucleic Acid
- From:Journal of Veterinary Science
2001;2(2):115-120
- CountryRepublic of Korea
- Language:English
-
Abstract:
This paper describes the cloning and sequence analysis of the cDNAs encoding the canine homologues of interleukin-3 (IL-3) and interleukin-6 (IL-6). The coding sequences for canine IL-3 and IL-6 were obtained by using the reverse transcription polymerase chain reaction (RT-PCR) with RNA harvested from canine peripheral blood mononuclear cells (PBMCs). Canine IL-3 cDNA includes a single open reading frame of 432 nucleotides, which encodes a 143 amino acid polypeptide and has 44.7, 42.4, 37 and 23.7% homology with the cow, sheep, human and rat IL-3 sequences, respectively. Canine IL-6 cDNA (GenBank accession number; AF275796) encodes a putative 20-amino acid signal peptide followed by a 187-amino acid mature protein. The predicted amino acid sequence of canine IL-6 shares 60.4, 77.2, 71.0, 55.8 and 42.0% sequence identity with those of human, feline, porcine, sheep and rat IL-6, respectively.
