The Study of Diagnostic Methods and Sequencing for Trichomoniasis in Mongolia

Giimaa Narantsogt; Burnee Mundurl; Zanabazar Enkhbaatar; Oyungerel Dogsom; Saruul Enkhbayar; Nyamaa Gunregjav; Urantsetseg Tsogtjarga; Narmandakh Dashdendev; Gurbadam Agvaandaram; Kyung-illm; Temuulen Dorjsuren

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The Study of Diagnostic Methods and Sequencing for Trichomoniasis in Mongolia

Author: Giimaa Narantsogt ^{1
,

2} ; Burnee Mundurl ³ ; Zanabazar Enkhbaatar ³ ; Oyungerel Dogsom ¹ ; Saruul Enkhbayar ¹ ; Nyamaa Gunregjav ⁴ ; Urantsetseg Tsogtjarga ⁴ ; Narmandakh Dashdendev ⁴ ; Gurbadam Agvaandaram ¹ ; Kyung-illm ⁵ ; Temuulen Dorjsuren ¹
Author Information

1. Department of Biology and Histology, School of Pharmacy and Biomedicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia
2. Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul, Republic of Korea
3. Department of Biology, Mongolian National University of Education, Ulaanbaatar, Mongolia
4. National Center for Communicable Diseases, Ulaanbaatar, Mongolia
5. Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul, Republic of Korea
Publication Type:Journal Article
Keywords: Sexually Transmitted Diseases; Trichomonas Vaginal is; Vaginitis; Polymerase Chain Reaction
From: Central Asian Journal of Medical Sciences 2015;1(1):41-48
CountryMongolia
Language:English
Abstract: Objectives: The aim of this study was to determine the usefulness of a diagnostic method for trichomoniasis that uses sequencing of the Tv-E650 gene. Methods: Totally 109 patients visited Ulaan Tuuz Hospital, National Center of Communicable Disease, Ulaanbaatar and were examined for trichomoniasis using wet mount examination, Papanicolaou (Pap) smear, culture and PCR. A family of 650 base pair (bp) long repeats from the T. vaginalis genome, designated as the Tv-E650 family, was sequenced for five Mongolian strains. Results: As a result, 21.1% (23/109) were positive by wet mount, 18.3% (20/109) were positive by Pap smear, 28.4% (31/109) were positive by culture and 36.6% (40/109) were positive by PCR. The differences among the strains are single-nucleotide and 2-nucleotide substitutions and insertions or deletions. The sequence uniformity of the strains as well as the presence of identical mutations in different isolates suggest efficient sequence homogenization mechanisms. In blast results, the Tv-E650 repeat family is conserved in all T. vaginalis strains examined, regardless of their diverse geographical origin. Conclusion: The Tv-E650 repeat family of T. vaginalis is a simple, rapid sensitive and specific accurate method for identification and diagnosis of trichomoniasis.