miR-495-3P targets BUB1 to regulate the effect of STAT3 signaling pathway on biological behavior of esophageal cancer cells
10.19405/j.cnki.issn1000-1492.2024.08.023
- Author:
Hui Yang
1
,
2
,
3
;
Ning Shi
4
;
Xiaowei Chen
1
,
2
,
3
;
Xuejie Song
1
,
2
,
3
;
Xi Zhou
1
,
2
,
3
;
Fuchun Si
1
,
2
,
3
Author Information
1. Traditional Chinese Medicine School，Henan University of Chinese Medicine，Zhengzhou 450046
2. Henan Key Laboratory of Traditional Chinese Medicine Syndrome and Prescription in Signaling，Zhengzhou 450046
3. Henan International Joint Laboratory of TCM Syndrome and Prescription in Signaling，Zhengzhou 450046
4. Medicine College of Henan University of Chinese Medicine，Zhengzhou 450046
- Publication Type:Journal Article
- Keywords:
esophageal cancer;
miR-495-3p;
BUB1;
STAT3 signaling pathway;
biological behavior
- From:
Acta Universitatis Medicinalis Anhui
2024;59(8):1446-1454
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effect of miR-495-3p targeting budding uninhibited by benzimidazoles (BUB1) on signal transducer and activator of transcription 3 (STAT3) signaling pathway on biological behavior of esophageal cancer (EC) cells．
Methods :The differentially expressed genes in EC tissues and normal tissues were screened by the cDNA microarray technique． The differentially expressed genes were analyzed by bioinformatics methods．The target genes of miRNAs were predicted by the TargetScan database and verified by a dual luciferase gene reporter assay．KYSE150 cells were divided into blank control group，NC mimics group and miR-495-3p mim- ics group．The activity of KYSE150 cells were detected by the CCK-8 method．Cell cycle and apoptosis were meas- ured by flow cytometry．The expression of BUB1 mRNA was measured by real-time fluorescence quantitative reverse transcription polymerase chain reaction (RT-qPCR) ．The levels of BUB1，STAT3，phosphor (p) -STAT3，cyclin dependent kinase 1 ( CCNB1) ，cyclin dependent kinase 1 ( CDK1) ，B-cell lymphoma-2 (Bcl-2) ，cysteinyl aspar- tate-specific proteinase-3 ( Caspase-3) and cysteinyl aspartate-specific proteinase-3 ( Caspase-9) were measured by Western blot．The migration and invasion abilities of the cells were measured by wound-healing and Transwell inva- sion assays.
Results :Differentially expressed genes were involved in biological processes，signaling pathways and network construction，which were mainly related to cell cycle．BUB1 is the key (Hub) gene，and BUB1 is the tar- get gene of miR-495-3p．In vitro experiments showed that overexpression of miR-495-3p could significantly inhibit the migration and invasion of EC cells and induce apoptosis and G2 / M phase arrest．After treatment with overex- pression of miR-495-3p，the expression of Caspase-3 and Caspase-9 in EC cells increased significantly(P＜0. 01) ， while the expression of Bcl-2，BUB1，CCNB1，CDK1 and p-STAT3 decreased significantly(P＜0. 01) ．Moreover， the STAT3 signaling pathway might play an important role in this process．
Conclusion :miR-495-3p may influence the biological behavior of esophageal cancer cells by down-regulating BUB1-mediated STAT3 signaling pathway.
- Full text:2024091415584005412miR-495-3p靶向B...食管癌细胞生物学行为的影响_杨晖.pdf
