Construction and validation of a gastric cancer prognosis model based on disulfidptosis-related LncRNAs
- VernacularTitle:双硫死亡相关LncRNA的胃癌预后模型构建与验证
- Author:
Liang FENG
1
;
Zhiguo CAO
;
Xiaoqi SHI
;
Qikun ZHANG
;
Changyu CHEN
;
Changjun YU
Author Information
- Keywords: long non-coding RNA; cell death; gastric cancer
- From: Acta Universitatis Medicinalis Anhui 2024;59(8):1429-1439
- CountryChina
- Language:Chinese
- Abstract: Objective Based on a novel type of cell death induced by disulfide stress,known as disulfidptosis,this study explores the role of long non-coding RNA(LncRNA)in gastric cancer and establishes a prognosis model re-lated to disulfidptosis,providing a new method for assessing the prognosis of gastric cancer treatment.Methods Transcriptomic data from gastric cancer and normal tissue samples were obtained from the public database TCGA,and disulfidptosis-related LncRNAs were selected through Pearson analysis and LASSO-Cox regression analysis.A relevant prognostic model for gastric cancer was constructed based on the above LncRNAs and validated by function-al enrichment analysis,tumour microenvironment and immune cell infiltration analysis,drug sensitivity analysis and quantitative reverse transcription PCR(RT-qPCR).Results In this study,400 disulfide death-associated LncR-NAs were identified and five of them were screened to construct a prognostic model for assessing the prognosis of gastric cancer patients.The models showed in validation that the survival of the high-risk score group was shorter than that of the low-risk score group(P＜0.05).In addition,the predictive ability of the prognostic model(AUC=0.725)was better than that based only on basic characteristics such as age and gender.The expression levels of disulfide death-associated LncRNAs differed between normal and gastric cancer tissues(P＜0.001).Conclusion The disulfidptosis-related LncRNA prognosis model developed in this study can effectively assess the prognosis of gastric cancer patients and the tumor microenvironment,providing potential targets and a theoretical basis for new immunotherapeutic strategies for gastric cancer.
- Full text:2024091415412896532双硫死亡相关LncRNA的胃癌预后模型构建与验证_凤亮.pdf
