Experimental study of isogenous micro-grafting technique combined with botulinum toxin type A in promoting hair regeneration
10.19405/j.cnki.issn1000-1492.2024.08.019
- VernacularTitle:同源细胞微移植技术联合A型肉毒毒素促进毛发再生的实验研究
- Author:
Qiuxuan ZHU
1
;
Caiying SONG
;
Shengrong CHENG
;
Wendong CHEN
;
Fei ZHU
Author Information
1. 安徽医科大学第一附属医院整形外科,合肥 230022
- Keywords:
hair follicle;
hair growth;
botulinum toxin type A;
micrograft
- From:
Acta Universitatis Medicinalis Anhui
2024;59(8):1417-1422
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of isogenous micro-grafting(MG)technology and the combined use of botulinum toxin type A(BTX-A)on hair regeneration in mice.Methods Forty-five C57BL/6 mice were randomly divided into five groups:normal saline group(NS group),MG group(MB-0 group),MG+low-dose BTX-A group(MB-2 group),MG+medium dose BTX-A group(MB-10 group),MG+high-dose BTX-A group(MB-50 group).The growth of hair on the backs of the mice was observed after the administration of the drugs,and the hair follicles were evaluated by hematoxylin-eosin(HE)staining on the 7th,14th,and 21st days of the experiment,and the expression of vascular endothelial growth factor(VEGF)and β-catenin was evaluated by immunohisto-chemical staining on day 21.Results After treatment with micrograft and botulinum toxin type A,the skin darke-ning time was shortened(P＜0.05),and the coverage rate of new hair increased on the 14 th day(P＜0.05).Compared with the NS group,the number of hair follicles increased(P＜0.05)and the expression of VEGF andβ-catenin increased in each other group.Conclusion Isogenous micro-grafting technology combined with botuli-num toxin type A has a promotional effect on hair regeneration in mice,and the mechanism may be related to the promotion of vascular growth and activation of β-catenin signaling.
