Experimental study of isogenous micro-grafting technique combined with botulinum toxin type A in promoting hair regeneration
10.19405/j.cnki.issn1000-1492.2024.08.019
- Author:
Qiuxuan Zhu
1
;
Caiying Song
1
;
Shengrong Cheng
1
;
Wendong Chen
1
;
Fei Zhu
1
Author Information
1. Dept of Plastic and Reconstructive Surgery， The First Affiliated Hospital of Anhui Medical University，Hefei 230022
- Publication Type:Journal Article
- Keywords:
hair follicle;
hair growth;
botulinum toxin type A;
micrograft
- From:
Acta Universitatis Medicinalis Anhui
2024;59(8):1417-1422
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the effects of isogenous micro-grafting(MG) technology and the combined use of botulinum toxin type A(BTX-A) on hair regeneration in mice．
Methods :Forty-five C57BL /6 mice were randomly divided into five groups : normal saline group (NS group) ，MG group (MB-0 group) ，MG + low-dose BTX-A group (MB-2 group) ，MG + medium dose BTX-A group ( MB-10 group ) ，MG + high-dose BTX-A group ( MB-50 group) ．The growth of hair on the backs of the mice was observed after the administration of the drugs，and the hair follicles were evaluated by hematoxylineosin ( HE) staining on the 7th ，14th，and 21st days of the experiment， and the expression of vascular endothelial growth factor ( VEGF) and β-catenin was evaluated by immunohisto- chemical staining on day 21 .
Results :After treatment with micrograft and botulinum toxin type A，the skin darke- ning time was shortened (P＜0. 05) ，and the coverage rate of new hair increased on the 14 th day (P＜0. 05) . Compared with the NS group，the number of hair follicles increased (P＜0. 05) and the expression of VEGF and β-catenin increased in each other group．
Conclusion :Isogenous micro-grafting technology combined with botulinum toxin type A has a promotional effect on hair regeneration in mice，and the mechanism may be related to the promotion of vascular growth and activation of β-catenin signaling.
- Full text:2024091415190272577同源细胞微移植技术联合A型...毒素促进毛发再生的实验研究_朱秋璇.pdf
