Effect of macrophages polarization on proliferation，migration and osteogenic differentiation of periodontal ligament stem cells
10.19405/j.cnki.issn1000-1492.2024.08.015
- Author:
Kepeng Li
1
,
2
;
Zhenguo Shen
1
;
Xiangdong Liu
3
;
Tiantian Cheng
3
;
Yuanyin Wang
1
Author Information
1. College and Hospital of Stomatology，Anhui Medical University，Key Lab of Oral Diseases Research of Anhui Province，Hefei 230032
2. Anqing 116 Hospital，Anqing 246004
3. Anqing 116 Hospital，Anqing 246004
- Publication Type:Journal Article
- Keywords:
macrophages;
periodontal ligament stem cells;
proliferation;
migration;
osteogenic differentiation
- From:
Acta Universitatis Medicinalis Anhui
2024;59(8):1392-1398
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the effects of different phenotypes macrophages (Mφs) on the proliferation，mi- gration and osteogenic differentiation of periodontal ligament stem cells (PDLSCs) ．
Methods :PDLSCs were isola- ted and cultured by tissue block method．Tohoku Hospital Pediatrics-1 (THP-1) cell line was stimulated to activate into unpolarized Mφs (M0) ，then induced to polarize into type I Mφs (M1) and type II Mφs (M2) ．Quantitative real-time PCR (qPCR) detected the inflammatory factors tumor necrosis factor α (TNF-α) ，interleukin (IL) -1 β , IL-6，IL-10 and transforming growth factor-β (TGF-β) mRNA expression level．After collecting culture superna- tants with different phenotypes，PDLSCs were stimulated，native control (NC) group did not receive the culture su- pernatant of Mφs．The effects of PDLSCs proliferation were assessed via Methylthiazolyldiphenyl-tetrazolium bro- mide (MTT) assay，while scratch assays were employed to evaluate their migration．Western blot was utilized to analyze the protein expression of Runt-related transcription factor 2 ( RUNX2) and alkaline phosphatase ( ALP) . Additionally，Alizarin Red staining was performed to investigate the deposition of calcified nodules in PDLSCs.
Results:qPCR showed the relative expression of TNF-α , IL-1 β and IL-6 in M1 Mφs were higher than those in M0 and M2 Mφs (P＜0. 05) ，and the relative expression of IL-10 and TGF-β in M2 Mφs were higher than those in M0 and M1 Mφs (P＜0. 05) ; Western blot showed the expression of RUNX2 and ALP proteins in PDLSCs in M0 and M2 groups was higher than those in the NC group (P ＜0. 05) ，Alizarin Red staining showed increased calcified nodule deposition in PDLSCs in M0，M1 and M2 groups compared to the NC group ; MTT assay showed the prolifer- ation of PDLSCs in the M0 and M1 groups was suppressed compared to the NC group (P＜0. 05) ; and scratch ex- periment showed the migratory capacity of PDLSCs in the M1 and M2 groups was stronger than that in the NC group．
Conclusion :M0 and M1 Mφs inhibit PDLSCs proliferation，M1 and M2 Mφs promote PDLSCs migration， and all types of Mφs promote osteogenic differentiation of PDLSCs.
- Full text:2024091415041815099巨噬细胞极化对牙周膜干细胞增殖、迁移、成骨分化的影响_李克朋.pdf
