Knockout SQLE in melanoma cells potentiates anti-tumor immunity via improving CD8 + T cell infiltration in tumor microenvironment
10.19405/j.cnki.issn1000-1492.2024.08.005
- Author:
Yao Ding
1
;
Wen Liu
2
;
Yiran Liao
1
;
Shun Lei
3
;
Yan Zhang
2
;
Yezi Chen
2
;
Yi Gong
1
;
Qizhao Huang
2
Author Information
1. Dept of Phase I Clinical Trial Ward，Chongqing University Cancer Hospital，Chongqing 400030
2. Institute of Immunological Innovation and Translation，Chongqing Medical University，Chongqing 400016
3. Institute of Immunology，Third Military Medical University，Chongqing 400038
- Publication Type:Journal Article
- Keywords:
squalene epoxidase;
melanoma;
tumor microenvironment;
tumor immunity
- From:
Acta Universitatis Medicinalis Anhui
2024;59(8):1315-1322
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the role of squalene epoxidase (SQLE) knockout in anti-tumor effect vial im- proving CD8 + T cell infiltration in melanoma tumor microenvironment．
Methods :Both immunodeficient and immu- nocompetent mice were inoculated with SQLE knockout B16F10 cells to determine the cell-autonomous and non-au- tonomous regulation of malignancy．Antibody blockade ，Luminex multiplex assays ，and flow cytometry were em- ployed to explore the impact of SQLE gene knockout on the secretion of cytokines / chemokines and immune cell in- filtration．Bioinformatics analysis was conducted to validate the correlation between SQLE expression and immune infiltration as well as clinical prognosis in melanoma patients.
Results :Compared with immunodeficient mice， SQLE knockout significantly inhibited melanoma proliferation in immunocompetent mice and prolonged their surviv- al．SQLE knockout induced the secretion of cytokines and chemokines from tumor cells，improved CD8 + T cell in- filtration in the tumor microenvironment，thereby potentiating anti-tumor immunity．Bioinformatics analysis sugges- ted a significant correlation between SQLE and its corresponding immune infiltration markers with the prognosis of melanoma patients．
Conclusion :SQLE regulates anti-tumor immunity by controlling cytokines and chemokines re- leasing in tumor microenvironment，thus holding promise as a novel tumor immunotherapy target and efficacy predic- tion molecular indicator.
- Full text:2024091411232113930SQLE敲除促进黑色素瘤肿..._T细胞浸润发挥抗肿瘤效应_丁瑶.pdf
