The expression levels and clinical significance of cold induced RNA binding protein and myeloid cell trigger receptor-1 in peripheral blood of patients with chronic obstructive pulmonary disease
10.19405/j.cnki.issn1000-1492.2024.07.026
- VernacularTitle:慢性阻塞性肺疾病患者外周血中冷诱导RNA结合蛋白和髓样细胞触发受体-1的表达水平及其临床意义
- Author:
Zheng ZHANG
1
;
Xueer CHEN
;
Xianling LU
Author Information
1. 石河子大学医学院内科学,石河子 832000
- Keywords:
chronic obstructive pulmonary disease;
cold-inducible RNA binding protein;
triggering receptor ex-pressed on myeloid cells-1
- From:
Acta Universitatis Medicinalis Anhui
2024;59(7):1275-1280
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression levels and clinical significance of cold-inducible RNA-binding protein(CIRBP)and triggering receptor expressed on myeloid cells-1(TREM-1)in peripheral blood of patients with chronic obstructive pulmonary disease(COPD).Methods Forty hospitalized COPD patients from October 2022 to June 2023 were selected as the acute exacerbation group.After treatment,they entered the stable phase and were included in the stable phase group.During the same period,40 healthy individuals underwent physical exami-nations as the control group.General information from each group was collected,peripheral blood was collected,and the levels of CIRBP and TREM-1 in plasma were measured using enzyme-linked immunosorbent assay(ELISA).The relative expression levels of CIRBP mRNA and TREM-1 mRNA were measured using real-time fluorescence quantitative polymerase chain reaction(qRT-PCR),and lung function was measured in COPD patients and healthy individuals.Results The expression levels of CIRBP and TREM-1 in peripheral blood during the acute exacerba-tion of COPD were higher than those in the stable phase group,and the differences were statistically significant(all P＜0.001);The expression levels of CIRBP and TREM-1 in peripheral blood during acute exacerbation and stable phases were higher than those in the control group,and the differences were statistically significant(all P＜0.001);In both acute exacerbation and stable phases,CIRBP levels were positively correlated with TREM-1 levels;The levels of CIRBP and TREM-1 during acute exacerbation were positively correlated with white blood cell count,neutrophil percentage,neutrophil to lymphocyte ratio;The stable CIRBP and TREM-1 levels were negatively correla-ted with the percentage of forced expiratory volume at 1 second to the expected value,and the ratio of forced expira-tory volume to forced vital capacity at 1 second.They were positively correlated with white blood cell count,neutro-phil percentage,and neutrophil/lymphocyte ratio.Conclusion The expression levels of CIRBP and TREM-1 are el-evated in the peripheral blood of COPD patients.The expression of CIRBP is correlated with TREM-1 expression,and is associated with clinical indicators such as lung function,white blood cell count,neutrophil percentage,neutro-phil to lymphocyte ratio,monocyte to high-density lipoprotein ratio,etc.,suggesting that CIRBP and TREM-1 may jointly participate in the occurrence and development of COPD.
