Effect of Xiaoyao Wan on CYP2E1 and FasL / TNF-α signal pathway in metabolic associated steatohepatitis
10.19405/j.cnki.issn1000-1492.2024.07.017
- Author:
Xinyue Zhang
1
;
Yuwei Zhang
1
;
Mengqi Li
1
;
Yanan Meng
1
;
Ruoyu Li
2
;
Yuchuan Miao
1
Author Information
1. Dept of Pathology，Shanxi University of Chinese Medicine，Jinzhong 030619
2. Dept of Encephalopathy，Shanxi University of Chinese Medicine Affiliated Hospital，Taiyuan 030000
- Publication Type:Journal Article
- Keywords:
Xiaoyao Wan;
metabolic associated steatohepatitis;
cytochrome P450 2E1;
factor-related apoptosis ligand;
tumor necrosis factor-α;
tumor growth factor-β1
- From:
Acta Universitatis Medicinalis Anhui
2024;59(7):1218-1224
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To study the mechanism of the treatment of metabolism associated steatohepatitis ( MASH) in rats with Xiaoyao Wan．
Methods :Twenty-four SD male rats were randomly divided into control group ( CON group，n = 8) and model group ( n = 8 ) ．The model group was given high-fat diet ，carbon tetrachloride ( CCl4 ) subcutaneous injection，hunger and satiety disorder and tail clamping for four weeks to establish the MASH model， and the rats were randomly divided into the MOD group and the Xiaoyao Wan group (XYW group) ，with 8 rats in each group．The rats of the XYW group were given Xiaoyao Wan，the other two groups were given normal saline. Four weeks after administration ，the serum biochemical indexes and oxidative stress indexes of rats in different groups were detected．The pathological sections of rat liver tissue were observed by hematoxylin-eosin ( HE) stai- ning and oil red O staining．The expression of cytochrome P450 2E1 ( CYP2E1) ，factor-related apoptosis ligand (FasL) ，tumor necrosis factor-α ( TNF-α) and tumor growth factor-β1 ( TGF-β1 ) in rat liver were detected by Western blot method．The relative contents of CYP2E1，FasL，TNF-α and TGF-β1 mRNA in liver tissue were de- tected by RT-qPCR.
Results :The general condition of rats in the XYW group was significantly improved compared with the MOD group ; the level of hepatic index was significant decrease compared with the MOD group (P＜0. 01) and the level of body mass index was significant increase compared with the MOD group (P ＜0. 01) ; the serum levels of triglyceride ，total cholesterol ，low －density lipoprotein cholesterol ，aspartate aminotransferase ，alanine aminotransferase，malondialdehyde，monocyte chemotactic protein-1，TNF-α , and interleukin (IL) -18 were signif- icantly reduced compared with the MOD group (all P＜0. 05) ，and the levels of high－density lipoprotein choles- terol，superoxide dismutase，and IL-10 were significantly elevated compared with the MOD group (all P＜0. 05) ; the content of lipid droplets in hepatocytes of rats was significantly reduced compared with the MOD group under light microscope ; the protein levels of CYP2E1，FasL，TNF-α and TGF-β1 in liver tissues were significantly re- duced compared with the MOD group ( all P ＜0. 05 ) ，and the relative contents of CYP2E1 ，FasL，TNF-α and TGF-β1 mRNA were significantly reduced compared with the MOD group ( all P ＜0. 05) ．
Conclusion :Xiaoyao Wan can regulate the expression of CYP2E1，FasL，TNF-α and TGF-β1 in the liver tissue of MASH rats by reduc- ing the accumulation of fat in the liver，so as to achieve the purpose of treating MASH.
- Full text:2024091410180621156逍遥丸对代谢相关脂肪性肝炎..._TNF-α信号通路的影响_张馨月.pdf
