Polydatin protects vascular endothelial cells by modifying the JAK2/STAT3 signaling pathway
10.19405/j.cnki.issn1000-1492.2024.07.014
- VernacularTitle:虎杖苷通过调节JAK2/STAT3信号通路改善血管内皮细胞损伤的研究
- Author:
Wenwen KONG
1
,
2
;
Huizhen WEI
;
Yuanying XU
;
Wenjun SHA
;
Jun LU
;
Tao LEI
Author Information
1. 安徽医科大学上海普陀中心临床学院,上海 200062
2. 安徽医科大学第五临床医学院,合肥 230032
- Keywords:
atherosclerosis;
human umbilical vein endothelial cells;
polydatin;
JAK2/STAT3 signaling pathway
- From:
Acta Universitatis Medicinalis Anhui
2024;59(7):1201-1205,1212
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protective effect of polydatin on lipopolysaccharide-induced injury of human umbilical vein vascular endothelial cells(HUVECs)through the protein Janus kinase 2(JAK2)/signal transducers and activators of transcription 3(STAT3)signaling pathway.Methods HUVECs were cultured in vitro,and 500 ng/ml LPS induced their injury and set as a model group;based on the model group,endothelial cells were inter-vened with different concentrations(10,20,and 40 μmol/L)of polydatin for 24 h,and set as polydatin low concentration group,polydatin medium concentration group,and polydatin high concentration group,respectively;a control group was set as another group.CCK-8,monocyte-endothelial cell adhesion,scratch and Transwell assays were used to detect cell viability,adhesion,migration and invasive ability;ELISA was used to detect interleukin-6(IL-6)and tumor necrosis factor-alpha(TNF-α)levels in the cell supernatant;Western blot was used to detect the expression of proteins related to the JAK2/STAT3 signaling pathway levels of JAK2/STAT3 signaling pathway relat-ed proteins.Results Compared with the control group,the model group showed decreased cell survival(P＜0.01),increased cell adhesion,migration and invasion(P＜0.001,P＜0.05,P＜0.01),increased levels of IL-6 and TNF-α in the cell supernatant(P＜0.001),and increased levels of phosphorylation of JAK2 and STAT3 pro-teins in the cells(P＜0.05).Compared with the model group,LPS damage to cells was attenuated after polydatin intervention,cell survival was increased in polydatin low-,medium-and high-concentration groups(P＜0.05),cell adhesion,migration,and invasion decreased(P＜0.05,P＜0.05,P＜0.001),IL-6 and TNF-α levels in cell supernatants decreased(P＜0.05),and the levels of cellular JAK2 and STAT3 protein phosphorylation lev-els decreased(P＜0.05).Conclusion Polydatin seems to reduce the inflammatory injury of human umbilical vein endothelial cells induced by LPS,reducing the secretion of inflammatory factors,and inhibiting the ability of cell ad-hesion,migration and invasion,which may be related to the down-regulation of JAK2/STAT3 signal pathway by polydatin.
