Hsa_circ_0052513 in Plasma Exosomes as A New Diagnostic Marker for Non-Small Cell Lung Cancer
10.3971/j.issn.1000-8578.2024.24.0110
- VernacularTitle:血浆外泌体hsa_circ_0052513在非小细胞肺癌中的表达及意义
- Author:
Lei ZHANG
1
;
Chenxi LI
;
Huiyong PENG
;
Dongwei ZHU
;
Jingsong XIE
Author Information
1. 210037 南京,南京中医药大学附属南京医院(南京市第二医院)检验科
- Keywords:
hsa_circ_0052513;
NSCLC;
Plasma;
Exosomes
- From:
Cancer Research on Prevention and Treatment
2024;51(9):744-749
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen differentially-expressed circRNA in plasma exosomes of patients with non-small cell lung cancer(NSCLC)and verify its diagnostic value for NSCLC.Methods The plasma exosomes of six patients with NSCLC and six healthy people were analyzed by circRNA sequencing.The expression of hsa_circ_0052513 in plasma exosomes of 60 NSCLC patients and 60 healthy controls was detected by qRT-PCR.The expression level of hsa_circ_0052513 in plasma exosomes of NSCLC patients before and after surgery was detected by qRT-PCR.The correlation between the expression of plasma exosomal hsa_circ_0052513 and clinical data of patients with NSCLC was statistically analyzed.Results Sequencing results showed that the expression of hsa_circ_0052513 in the plasma exosomes of NSCLC patients was higher than that of healthy controls,and the results were confirmed by qRT-PCR.Hsa_circ_0052513 was decreased in patients with NSCLC after tumor resection(P<0.05).The high expression of plasma exosomal hsa_circ_0052513 in patients with NSCLC was correlated with tumor size,distant metastasis,and TNM stage of NSCLC(all P<0.05).The area under the curve of plasma exosomal hsa_circ_0052513 in the diagnosis of patients with NSCLC was 0.7904(P<0.0001).Conclusion Hsa_circ_0052513 is highly expressed in the plasma exosomes of patients with NSCLC and is related to tumor size,metastasis,and TNM stage.Hence,Hsa_circ_0052513 could be a new diagnostic marker for NSCLC.
