Effect of SV2B Overexpression on Biological Behavior of Glioblastoma and Its Mechanism
10.3971/j.issn.1000-8578.2024.24.0225
- VernacularTitle:SV2B过表达对胶质母细胞瘤生物学行为的影响及其机制
- Author:
Qian CHEN
1
;
Changyu XU
;
Qining CHEN
;
Hubin DUAN
Author Information
1. 030001 太原,山西医科大学第一临床医学院神经外科
- Keywords:
Glioblastoma;
SV2B;
Tumor prognosis;
Malignant progression
- From:
Cancer Research on Prevention and Treatment
2024;51(9):737-743
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the effect of SV2B overexpression on the growth,invasion,and apoptosis of glioblastoma cells,and to explore its potential mechanism.Methods We transfected glioblastoma u87 and u251 cells with lentivirus as SV2B overexpression group.And blank control group was set up.The effects of SV2B overexpression on the proliferation,migration,and invasion of u87 and u251 cells were detected by CCK-8 assay,cell scratch assay,Transwell invasion,and Transwell migration assay.The expression level of SV2B protein was detected by qRT-PCR and Western blot.Results Compared with the blank control group,the proliferation,invasion,and migration abilities of u87 and u251 cells in SV2B overexpression group were significantly reduced(P<0.05).Conclusion SV2B overexpression significantly inhibits the proliferation,invasion,and migration abilities of glioblastoma cells.
