In Vitro Experimental Methods in Pharmacological Research of Chinese Materia Medica： A Review
10.13422/j.cnki.syfjx.20240740
- VernacularTitle:中药药理学体外实验方法研究进展
- Author:
Qiuju ZHANG
1
;
Nenglian LI
1
;
Yu WANG
1
;
Hua JIN
1
Author Information
1. Gansu University of Chinese Medicine， Lanzhou 730000， China
- Publication Type:Journal Article
- Keywords:
pharmacology of Chinese materia medica;
in vitro experiment;
drug-containing serum/plasma;
drug-containing intestinal absorption solution;
drug-containing liver incubation solution
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2024;30(20):283-289
- CountryChina
- Language:Chinese
-
Abstract:
In vitro experiments are an indispensable method in the pharmacological research of Chinese materia medica. However， the development of in vitro experiments is hindered by the various sources， complex composition， and unstable physical and chemical properties of Chinese materia medica. Therefore， it has become an urgent problem to develop reasonable methods for in vitro pharmacological research. At present， the commonly used in vitro experimental methods in the pharmacological research of Chinese materia medica include direct administration and indirect administration. Indirect administration methods include drug-containing serum/plasma， drug-containing intestinal absorption solution， and drug-containing liver incubation solution. With the development of computer technology and systems biology， the combination of high performance liquid chromatography-mass spectrometry （HPLC-MS）， omics， network pharmacology， computer/artificial intelligence-assisted drug design and other technologies with in vitro experiments of Chinese materia medica has not only greatly reduced the research cost but also significantly improved the efficiency of pharmacological exploration， effective ingredient screening， and drug discovery. Therefore， the combined methods have been gradually recognized and widely used. This paper reviewed the in vitro experimental methods in pharmacological research of Chinese materia medica and their progress， aiming to provide help for selecting reasonable research methods.
