Mechanism of Action of Coptidis Rhizoma and Ophiopogonis Radix in Delaying Diabetic Nephropathy Based on EGFR/PI3K/Akt Signaling Pathway
10.13422/j.cnki.syfjx.20241202
- VernacularTitle:基于EGFR/PI3K/Akt信号通路探讨黄连-麦冬药对延缓糖尿病肾病的作用机制
- Author:
Shaoyu LI
1
;
Man GONG
1
;
Qiufang LI
2
;
Liping DAI
1
;
Guiqun WANG
1
;
Qiuchen YANG
1
;
Qiongqiong ZHANG
1
;
Erping XU
1
;
Yalin LIU
1
Author Information
1. Henan Collaborative Innovation Center for Research and Development on the Whole Industry Chain of Yu-Yao，Henan University of Chinese Medicine，Zhengzhou 450046，China
2. Reproductive Health Hospital of the Third Affiliated Hospital of Zhengzhou University，Zhengzhou 450046，China
- Publication Type:Journal Article
- Keywords:
Coptidis Rhizoma;
Ophiopogonis Radix;
type 2 diabetes;
diabetic nephropathy;
epidermal growth factor receptor （EGFR）/phosphatidylinositol 3-kinase （PI3K）/protein kinase B （Akt） signaling pathway
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2024;30(20):22-29
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the effect of Coptidis Rhizoma and Ophiopogonis Radix on renal tissue injury and epidermal growth factor receptor （EGFR）/phosphatidylinositol 3-kinase （PI3K）/protein kinase B （Akt） signaling pathway in rats with diabetic nephropathy （DN） and explore its possible mechanism of delaying DN. MethodThirty-six male Wistar rats were randomly divided into a normal group （6 rats） and a model group （30 rats）. The model group was fed with a high-fat and high-sugar diet combined with streptozotocin （STZ） to establish a rat model of type 2 diabetes. After the successful preparation of the model， the rats were randomly divided into the model group， low， medium， and high dose groups of Coptidis Rhizoma and Ophiopogonis Radix （100， 200， 400 mg·kg-1）， and metformin group （200 mg·kg-1）. After administration， the levels of fasting blood glucose （FBG）， 24 h urine protein （24 h-UTP）， creatinine （SCr）， urea nitrogen （BUN）， and uric acid （UA） were detected. Hematoxylin-eosin （HE） staining and Masson staining were used to observe the pathological changes of renal tissue in rats. Western blot and Real-time fluorescence quantitative polymerase chain reaction （Real-time PCR） were used to detect the related protein expression of EGFR， PI3K， and Akt and their mRNA expression levels in the renal tissue of rats in each group. ResultsCompared with the normal group， the levels of FBG， SCr， BUN， UA， 24 h-UTP， and kidney index in the model group were significantly increased （P<0.01）， most renal tubular epithelial cells were necrotic， and the content of collagen in glomeruli was significantly increased （P<0.01）. Compared with the model group， the above indexes of rats in each administration group were improved to varying degrees. The FBG， SCr， BUN， UA， 24 h-UTP， and kidney index of rats in each dose group and metformin group were significantly decreased （P<0.01， P<0.05）. The necrosis degree of renal tubular epithelial cells was reduced， and the fibrosis area was decreased （P<0.01）. There related protein and mRNA expressions of EGFR， PI3K， and Akt were significantly increased （P<0.05， P<0.01）. ConclusionCoptidis Rhizoma and Ophiopogonis Radix can alleviate renal tissue injury in rats with DN， and their mechanism may be related to the regulation of the EGFR/PI3K/Akt signaling pathway.
