Bioinformatics analysis of HBV s gene mutation in blood donor

VernacularTitle:献血者HBVs基因变异的生物信息学分析
Author: Jiacheng FAN ¹ ; Qing LI ; Xiuli CHEN ; Shixiang LIU ; Ye CHEN
Author Information

1. 泰安市中心血站,山东泰安 271000
Keywords: HBV; OBI; s gene; blood donor; bioinformatics
From: Chinese Journal of Blood Transfusion 2024;37(8):933-939
CountryChina
Language:Chinese
Abstract: Objective To analyze the infectious indicators,HBV s gene mutations and changes in bioinformatics char-acteristics of HBV DNA+/HBsAg-blood donor.Methods A sample of HBV DNA+/HBsAg-was screened by PCR meth-od,and HBsAg/HBsAb/HBeAg/HBeAb/HBcAb of HBV in the sample were detected by ELISA and chemiluminescence methods.The mutation of the HBVs gene fragment in the sample was sequenced and analyzed,and the bioinformatics analy-sis was performed using analysis software.Results The sample showed HBV DNA+,HBsAg-,HBsAb+,HBeAg+,HBe-Ab-and HBcAb+,with an amino acid unit point mutation(P151L)occurred in the HBV s gene,and the spatial structure changed.Conclusion The spatial structural changes in the gene sequence of HBVs may be the reason for the appearance of serological HBsAg-/HBsAb+/HBV DNA+in the test results.